Featured Product
KD/KO Validated

Glucocorticoid receptor Polyclonal antibody

Name: Glucocorticoid receptor Polyclonal antibody
Brand: Proteintech
SKU: 24050-1-AP
Rating: 4.25 (4 reviews)

Glucocorticoid receptor Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (4)

Cat no : 24050-1-AP

Synonyms

NR3C1, GRL, GR, GCR, GCCR

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 24050-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 24050-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 24050-1-AP
WB result of Glucocorticoid receptor antibody (24050-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glucocorticoid receptor transfected HepG2 cells.
WB analysis using 24050-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 24050-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 24050-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 24050-1-AP
IP result of anti-Glucocorticoid receptor (IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:4000) with HepG2 cells lysate 1720 ug.
IP experiment of HepG2 using 24050-1-AP
IP result of anti-Glucocorticoid receptor (IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:4000) with HepG2 cells lysate 1720 ug.
IP experiment of mouse heart using 24050-1-AP
IP result of anti-Glucocorticoid receptor (IP:24050-1-AP, 4ug; Detection:24050-1-AP 1:500) with mouse heart tissue lysate 4400ug.
IHC staining of human prostate cancer using 24050-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 24050-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 24050-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 24050-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24050-1-AP (Glucocorticoid receptor antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 24050-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using Glucocorticoid receptor antibody (24050-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 24050-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (24050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 24050-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Glucocorticoid receptor antibody (24050-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

-/ -

Cat No. 24050-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, DU 145 cells, mouse brain tissue, rat brain tissue, PC-12 cells
Positive IP detected in	HepG2 cells, mouse heart tissue
Positive IHC detected in	human prostate cancer tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 64 publications below
IHC	See 6 publications below
IF	See 16 publications below
IP	See 2 publications below
CoIP	See 5 publications below
ChIP	See 17 publications below

Product Information

24050-1-AP targets Glucocorticoid receptor in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, zebrafish, rare minnow
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Glucocorticoid receptor fusion protein Ag21146 相同性解析による交差性が予測される生物種
Full Name	nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Calculated molecular weight	86 kDa
Observed molecular weight	94-97 kDa
GenBank accession number	BC015610
Gene symbol	Glucocorticoid receptor
Gene ID (NCBI)	2908
RRID	AB_2813890
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).

Protocols

Product Specific Protocols
WB protocol for Glucocorticoid receptor antibody 24050-1-AP	Download protocol
IHC protocol for Glucocorticoid receptor antibody 24050-1-AP	Download protocol
IF protocol for Glucocorticoid receptor antibody 24050-1-AP	Download protocol
IP protocol for Glucocorticoid receptor antibody 24050-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Metab Individual-specific functional epigenomics reveals genetic determinants of adverse metabolic effects of glucocorticoids. Authors - Wenxiang Hu View Article
mouse	IHC	Cell Metab A macrophage-hepatocyte glucocorticoid receptor axis coordinates fasting ketogenesis. Authors - Anne Loft View Article
human	WB	Sci Adv Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment. Authors - Leiming Wang View Article
mouse	chIP	Mol Cell Cistromic Reprogramming of the Diurnal Glucocorticoid Hormone Response by High-Fat Diet. Authors - Fabiana Quagliarini View Article
mouse	WB,ChIP,IP	J Clin Invest REVERBa couples the circadian clock to hepatic glucocorticoid action. Authors - Giorgio Caratti View Article
mouse	ChIP	Mol Psychiatry The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice. Authors - Alexander S Häusl View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Ping (Verified Customer) (06-04-2019)	This antibody works well for immunoprecipitation and western blot. Applications: Western Blot, Immunoprecipitation, Cell Tissue Type: mouse brain tissue
FH Yu Siong (Verified Customer) (04-23-2019)	Multiple bands observed - Total of 5 bands, not suitable for porcine brain endothelial cells, as non-specific binding tend to occur.Consistent in two Independent experiments Applications: Western Blot, Primary Antibody Dilution: 1:500 Cell Tissue Type: Porcine brain endothelial cells
FH Praveen (Verified Customer) (02-25-2019)	WORKS VERY WELL. Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Cell culture, ChIP, Cell Tissue Type: Ac16
FH Shubham (Verified Customer) (01-31-2019)	Antibody works great. Applications: Western Blot, Immunohistochemistry, Cell culture, Primary Antibody Dilution: 1:10 Cell Tissue Type: Ac16 Glucocorticoid receptor Antibody Western Blot,Immunohistochemistry,Cell culture, validation (1:10 dilution) in Ac16 (Cat no:24050-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Glucocorticoid receptor Polyclonal antibody

Glucocorticoid receptor Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 24050-1-AP

WB analysis using 24050-1-AP

WB analysis of HepG2 using 24050-1-AP

WB analysis using 24050-1-AP

WB analysis using 24050-1-AP

WB analysis using 24050-1-AP

IP experiment of HepG2 using 24050-1-AP

IP experiment of HepG2 using 24050-1-AP

IP experiment of mouse heart using 24050-1-AP

IHC staining of human prostate cancer using 24050-1-AP

IHC staining of human prostate cancer using 24050-1-AP

IHC staining of human ovary tumor using 24050-1-AP

IHC staining of human ovary tumor using 24050-1-AP

IF Staining of A549 using 24050-1-AP

IF Staining of HepG2 using 24050-1-AP

IF Staining of HepG2 using 24050-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Individual-specific functional epigenomics reveals genetic determinants of adverse metabolic effects of glucocorticoids.

A macrophage-hepatocyte glucocorticoid receptor axis coordinates fasting ketogenesis.

Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.

Cistromic Reprogramming of the Diurnal Glucocorticoid Hormone Response by High-Fat Diet.

REVERBa couples the circadian clock to hepatic glucocorticoid action.

The co-chaperone Fkbp5 shapes the acute stress response in the paraventricular nucleus of the hypothalamus of male mice.

Reviews

Related Products

HSP90 Polyclonal antibody

PR Monoclonal antibody

androgen receptor Polyclonal antibody

NR3C2 Polyclonal antibody

BAG1 Polyclonal antibody

ER Polyclonal antibody

NF-κB p65 Polyclonal antibody

FKBP52 Polyclonal antibody

NFKB1,p105,p50 Polyclonal antibody