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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NR1D1 Polyclonal antibody (14506-1-AP)

    WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NR1D1 Polyclonal antibody (14506-1-AP)

    WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NR1D1 Polyclonal antibody (14506-1-AP)

    WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NR1D1 Polyclonal antibody (14506-1-AP)

    WB analysis using 14506-1-AP (same clone as 14506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14506-1-AP (NR1D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using NR1D1 Polyclonal antibody (14506-1-AP)

    WB analysis of A549 cells using 14506-1-AP (same clone as 14506-1-PBS)

    WB result of anti-NR1D1 (14506-1-AP,1:1000) with A549 cell by Dr. Toryn Poolman. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using NR1D1 Polyclonal antibody (14506-1-AP)

    WB analysis of A549 cells using 14506-1-AP (same clone as 14506-1-PBS)

    WB result of anti-NR1D1 (14506-1-AP,1:500) with A549 treated with Cycloheximide by Dr. Toryn Poolman. This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using NR1D1 Polyclonal antibody (14506-1-AP)

    IHC staining of mouse skeletal muscle using 14506-1-AP (same clone as 14506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14506-1-AP (NR1D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using NR1D1 Polyclonal antibody (14506-1-AP)

    IHC staining of mouse skeletal muscle using 14506-1-AP (same clone as 14506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14506-1-AP (NR1D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using NR1D1 Polyclonal antibody (14506-1-AP)

    IF Staining of NIH/3T3 using 14506-1-AP (same clone as 14506-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 14506-1-AP (NR1D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14506-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NR1D1 Polyclonal antibody, PBS Only

NR1D1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14506-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ear 1, EAR1, EAR-1, HREV, Rev erbA alpha

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14506-1-PBS targets NR1D1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5964

Product name: Recombinant human NR1D1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC056148

Sequence: MTTLDSNNNTGGVITYIGSSGSSPSRTSPESLYSDNSNGSFQSLTQGCPTYFPPSPTGSLTQDPARSFGSIPPSLSDDGSPSSSSSSSSSSSSFYNGSPPGSLQVAMEDSSRVSPSKSTSNITKLNGMVLLCKVCGDVASGFHYGVHACEGCKGFFRRSIQQNIQYKRCLKNENCSIVRINRNRCQQCRFKKCLSVGMSRDAVRFGRIPKREKQRMLAEMQSAMNLANNQLSSQCPLETSPTQHPTPGPMGPSPPPAPVPSPLVGFSQFPQQLTPPRSPSPEPTVEDVISQVARAHREIFTYAHDKLGSSPGNFNANHASGSPPATTPHRWENQGCPPAPNDNNTLAAQR

相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 1, group D, member 1
Calculated molecular weight 67 kDa
Observed molecular weight68 kDa
GenBank accession numberBC056148
Gene Symbol NR1D1
Gene ID (NCBI) 9572
RRIDAB_11182604
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP20393
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NR1D1, also named EAR1, THRAL, ear-1, and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. BMAL1-CLOCK activates Rev-erba itself and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.

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