Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse spinal cord tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30848-1-AP targets NPY5R in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5428 Product name: Recombinant human NPY5R protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 240-382 aa of BC042416 Sequence: CGLSNKENRLEENEMINLTLHPSKKSGPQVKLSGSHKWSYSFIKKHRRRYSKKTACVLPAPERPSQENHSRILPENFGSVRSQLSSSSKFIPGVPTCFEIKPEENSDVHELRVKRSVTRIKKRSRSVFYRLTILILVFAVSWM 相同性解析による交差性が予測される生物種 |
| Full Name | neuropeptide Y receptor Y5 |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC042416 |
| Gene Symbol | NPY5R |
| Gene ID (NCBI) | 4889 |
| RRID | AB_3742376 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q15761 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Neuropeptide Y receptor Y5 (NPY5R) is a G protein-coupled receptor (GPCR) that belongs to the neuropeptide Y receptor family and is primarily involved in regulating food intake, energy metabolism, and various physiological processes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NPY5R antibody 30848-1-AP | Download protocol |
| IHC protocol for NPY5R antibody 30848-1-AP | Download protocol |
| WB protocol for NPY5R antibody 30848-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |