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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NPTXR Recombinant monoclonal antibody (85994-1-RR)

    WB analysis using 85994-1-RR (same clone as 85994-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85994-1-RR (NPTXR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85994-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NPTXR Recombinant monoclonal antibody (85994-1-RR)

    WB analysis using 85994-1-RR (same clone as 85994-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 85994-1-RR (NPTXR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85994-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NPTXR Recombinant monoclonal antibody (85994-1-RR)

    IHC staining of mouse brain using 85994-1-RR (same clone as 85994-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85994-1-RR (NPTXR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85994-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NPTXR Recombinant monoclonal antibody (85994-1-RR)

    IHC staining of mouse brain using 85994-1-RR (same clone as 85994-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85994-1-RR (NPTXR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85994-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NPTXR Recombinant monoclonal antibody (85994-1-RR)

    IF Staining of mouse brain using 85994-1-RR (same clone as 85994-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NPTXR antibody (85994-1-RR, Clone: 250488B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85994-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NPTXR Recombinant monoclonal antibody (85994-1-RR)

    IF Staining of mouse brain using 85994-1-RR (same clone as 85994-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NPTXR antibody (85994-1-RR, Clone: 250488B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85994-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85994-1-RR

    Affinity and Kinetic Characterization of 85994-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85994-1-RR against Human NPTXR were performed. The affinity constant is 0.161 nM.

NPTXR Recombinant monoclonal antibody, PBS Only

NPTXR Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 85994-1-PBS
Clone No.250488B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Neuronal pentraxin receptor

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85994-1-PBS targets NPTXR in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg3109

Product name: Recombinant Human NPTXR protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 24-500 aa of NM_014293.3

Sequence: ASVPLAASPARALPGGADNASVASGAAASPGPQRSLSALHGAGGSAGPPALPGAPAASAHPLPPGPLFSRFLCTPLAAACPSGAQQGDAAGAAPGEREELLLLQSTAEQLRQTALQQEARIRADQDTIRELTGKLGRCESGLPRGLQGAGPRRDTMADGPWDSPALILELEDAVRALRDRIDRLEQELPARVNLSAAPAPVSAVPTGLHSKMDQLEGQLLAQVLALEKERVALSHSSRRQRQEVEKELDVLQGRVAELEHGSSAYSPPDAFKISIPIRNNYMYARVRKALPELYAFTACMWLRSRSSGTGQGTPFSYSVPGQANEIVLLEAGHEPMELLINDKVAQLPLSLKDNGWHHICIAWTTRDGLWSAYQDGELQGSGENLAAWHPIKPHGILILGQEQDTLGGRFDATQAFVGDIAQFNLWDHALTPAQVLGIANCTAPLLGNVLPWEDKLVEAFGGATKAAFDVCKGRAKA

相同性解析による交差性が予測される生物種
Full Name neuronal pentraxin receptor
Calculated molecular weight53 kDa
Observed molecular weight35-65 kDa
GenBank accession numberNM_014293.3
Gene Symbol NPTXR
Gene ID (NCBI) 23467
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO95502
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NPTXR (Neuronal Pentraxin Receptor) is a type II transmembrane glycoprotein primarily expressed in the cerebellum and hippocampus. It mediates synaptic plasticity by clustering AMPA-type glutamate receptors at excitatory synapses and promoting synaptogenesis. NPTXR can be proteolytically cleaved to release a soluble form. Its dysregulation is implicated in Alzheimer's disease and small-cell lung cancer.(PMID: 9261167; 18367087; 19368810; 16115696)

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