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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NPTX2 Monoclonal antibody (68709-1-Ig)

    WB analysis using 68709-1-Ig (same clone as 68709-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68709-1-Ig (NPTX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68709-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NPTX2 Monoclonal antibody (68709-1-Ig)

    IHC staining of mouse brain using 68709-1-Ig (same clone as 68709-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68709-1-Ig (NPTX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68709-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NPTX2 Monoclonal antibody (68709-1-Ig)

    IHC staining of mouse brain using 68709-1-Ig (same clone as 68709-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68709-1-Ig (NPTX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68709-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using NPTX2 Monoclonal antibody (68709-1-Ig)

    IF Staining of SH-SY5Y using 68709-1-Ig (same clone as 68709-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NPTX2 antibody (68709-1-Ig, Clone: 2H8D8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68709-1-PBS in a different storage buffer formulation.

NPTX2 Monoclonal antibody, PBS Only

NPTX2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68709-1-PBS
Clone No.2H8D8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

2H8D8, Neuronal pentraxin II, Neuronal pentraxin-2, NP2, NP-II

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68709-1-PBS targets NPTX2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag33808

Product name: Recombinant human NPTX2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC009924

Sequence: MLALLAASVALAVAAGAQDSPAPGSRFVCTALPPEAVHAGCPLPAMPMQGGAQSPEEELRAAVLQLRETVVQQKETLGAQREAIRELTGKLARCEGLAGGKARGAGATGKDTMGDLPRDPGHVVEQLSRSLQTLKDRLESLEHQLRANVSNAGLPGDFREVLQQRLGELERQLLRKVAELEDEKSLLHNETSAHRQKTESTLNALLQRVTELERGNSAFKSPDAFKVSLPLRTNYLYGKIKKTLPELYAFTICLWLRSSASPGIGTPFSYAVPGQANEIVLIEWGNNPIELLINDKVAQLPLFVSDGKWHHICVTWTTRDGMWEAFQDGEKLGTGENLAPWHPIKPGGVL

相同性解析による交差性が予測される生物種
Full Name neuronal pentraxin II
Calculated molecular weight 47 kDa
Observed molecular weight50-55 kDa
GenBank accession numberBC009924
Gene Symbol NPTX2
Gene ID (NCBI) 4885
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP47972
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is a secreted protein involved in excitatory synapse formation. It also plays a role in the clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)

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