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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NPTX2 Polyclonal antibody (10889-1-AP)

    WB analysis using 10889-1-AP (same clone as 10889-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using NPTX2 Polyclonal antibody (10889-1-AP)

    WB analysis of SH-SY5Y using 10889-1-AP (same clone as 10889-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using NPTX2 Polyclonal antibody (10889-1-AP)

    WB analysis of PC-3 using 10889-1-AP (same clone as 10889-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10889-1-AP (NPTX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NPTX2 Polyclonal antibody (10889-1-AP)

    IP experiment of mouse brain using 10889-1-AP (same clone as 10889-1-PBS)

    IP result of anti-NPTX2 (IP:10889-1-AP, 4ug; Detection:10889-1-AP 1:600) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using NPTX2 Polyclonal antibody (10889-1-AP)

    IHC staining of human brain using 10889-1-AP (same clone as 10889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using NPTX2 Polyclonal antibody (10889-1-AP)

    IHC staining of human brain using 10889-1-AP (same clone as 10889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NPTX2 Polyclonal antibody (10889-1-AP)

    IHC staining of human pancreas cancer using 10889-1-AP (same clone as 10889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10889-1-AP (NPTX2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NPTX2 Polyclonal antibody (10889-1-AP)

    IHC staining of human pancreas cancer using 10889-1-AP (same clone as 10889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NPTX2 Polyclonal antibody (10889-1-AP)

    IHC staining of human pancreas cancer using 10889-1-AP (same clone as 10889-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10889-1-AP (NPTX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using NPTX2 Polyclonal antibody (10889-1-AP)

    IF Staining of SH-SY5Y using 10889-1-AP (same clone as 10889-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NPTX2 antibody (10889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10889-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NPTX2 Polyclonal antibody, PBS Only

NPTX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10889-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Neuronal pentraxin II, Neuronal pentraxin-2, NP2, NP-II

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10889-1-PBS targets NPTX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1326

Product name: Recombinant human NPTX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC009924

Sequence: MLALLAASVALAVAAGAQDSPAPGSRFVCTALPPEAVHAGCPLPAMPMQGGAQSPEEELRAAVLQLRETVVQQKETLGAQREAIRELTGKLARCEGLAGGKARGAGATGKDTMGDLPRDPGHVVEQLSRSLQTLKDRLESLEHQLRANVSNAGLPGDFREVLQQRLGELERQLLRKVAELEDEKSLLHNETSAHRQKTESTLNALLQRVTELERGNSAFKSPDAFKVSLPLRTNYLYGKIKKTLPELYAFTICLWLRSSASPGIGTPFSYAVPGQANEIVLIEWGNNPIELLINDKVAQLPLFVSDGKWHHICVTWTTRDGMWEAFQDGEKLGTGENLAPWHPIKPGGVL

相同性解析による交差性が予測される生物種
Full Name neuronal pentraxin II
Calculated molecular weight 47 kDa
Observed molecular weight 47-50 kDa
GenBank accession numberBC009924
Gene Symbol NPTX2
Gene ID (NCBI) 4885
RRIDAB_2153875
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP47972
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Neuronal pentraxins constitute a family of proteins that are homologous to C-reactive protein (CRP) and serum amyloid P component (SAP), including NPTX1, NPTX2, and the neuronal pentraxin receptor (NPTXR). NPTX2, also known as NARP (neuronal activity-regulated pentaxin), is a secreted protein involved in excitatory synapse formation. It also plays a role in the clustering of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors at established synapses, resulting in non-apoptotic cell death of dopaminergic nerve cells. NPTX2 is highly up-regulated in Parkinson's disease (PD), suggesting it may be involved in the pathology of PD. (PMID: 12895424; 17987278; 10748068)

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