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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human kidney tissue using NPVF Polyclonal antibody (22311-1-AP)

    IHC staining of human kidney using 22311-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22311-1-AP (NPSF antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NPVF Polyclonal antibody (22311-1-AP)

    IHC staining of human kidney using 22311-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22311-1-AP (NPSF antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hypothalamus tissue using NPVF Polyclonal antibody (22311-1-AP)

    IHC staining of human hypothalamus using 22311-1-AP

    Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 22311-1-AP (NPSF antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hypothalamus tissue using NPVF Polyclonal antibody (22311-1-AP)

    IHC staining of human hypothalamus using 22311-1-AP

    Immunohistochemical analysis of paraffin-embedded human hypothalamus tissue slide using 22311-1-AP (NPSF antibody at dilution of 1:200 (under 40x lens).

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NPVF Polyclonal antibody

NPVF Polyclonal Antibody for IHC, ELISA
Cat No. 22311-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

C7orf9, FMRFamide related peptides, Neuropeptide RFRP 3, Neuropeptide VF, neuropeptide VF precursor, NPSF, NPVF, RFRP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman kidney tissue, human hypothalamus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22311-1-AP targets NPVF in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name neuropeptide VF precursor
Calculated molecular weight 22 kDa
GenBank accession numberNM_022150
Gene Symbol NPVF
Gene ID (NCBI) 64111
RRIDAB_3669395
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9HCQ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human RFamide-related peptide gene, RFRP (also designated NPVF or C7orf9), is responsible for encoding three small neuropeptides designated NPSF, RFRP-1 RFRP-2 and RFRP-3 (NPVF). Neuropeptide RFRP-1 acts as a potent negative regulator of gonadotropin synthesis and secretion. Neuropeptides NPSF and NPVF efficiently inhibit forskolin-induced production of cAMP, but RFRP-2 shows no inhibitory activity. Neuropeptide RFRP-1 induces secretion of prolactin in rats. Neuropeptide NPVF blocks morphine-induced analgesia. This antibody can recognize neuropeptide VF precursor and neuropeptide NPSF.

Protocols

Product Specific Protocols
IHC protocol for NPVF antibody 22311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols