Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, LNCaP cells, THP-1 cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26446-1-AP targets NPRL3 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24045 Product name: Recombinant human C16orf35 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-390 aa of BC004185 Sequence: MADGNEGPQSPFHHILPKCKLARDLKEAYDSLCTSGVVRLHINSWLEVSFCLPHKIHYAASSLIPPEAIERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALVRVIKTTSAVKNLQQLAQDADLALLQVFQLAAHLVYWGKAIIIYPLCENNVYMLSPNASVCLYSPLAEQFSHQFPSHDLPSVLAKFSLPVSLSEFRNPLAPAVQETQLIQMVVWMLQRRLLIQLHTYVCLMASPSEEEPRPREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQRMTENLLASLSEHERAAILSVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQLLMLFDKFRSVLVVTTHEDPVIAVFQALLP 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 16 open reading frame 35 |
| Calculated molecular weight | 64 kDa |
| Observed molecular weight | 63 kDa |
| GenBank accession number | BC004185 |
| Gene Symbol | C16orf35 |
| Gene ID (NCBI) | 8131 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12980 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NPRL3 (Nitrogen Permease Regulator Like 3) is a highly conserved tumor suppressor protein. As a core component of the GATOR1 complex, it serves as a key node in the amino acid sensing pathway and negatively regulates the mTORC1 signaling pathway, thereby controlling cell growth, proliferation, and autophagy. Loss of its function leads to hyperactivation of mTORC1 signaling. In neuroscience, loss-of-function mutations in NPRL3 are an important genetic cause of familial focal epilepsy, such as focal epilepsy with variable foci. In oncology, it is frequently inactivated through epigenetic silencing in various cancers, making it a significant tumor suppressor and a potential biomarker.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NPRL3 antibody 26446-1-AP | Download protocol |
| WB protocol for NPRL3 antibody 26446-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |