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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NPR1 Recombinant antibody (84043-1-RR)

    WB analysis using 84043-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84043-1-RR (NPR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NPR1 Recombinant antibody (84043-1-RR)

    IF Staining of HeLa using 84043-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NPR1 antibody (84043-1-RR, Clone: 240696A9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

NPR1 Recombinant antibody

NPR1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84043-1-RR
Clone No.240696A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

GC A, ANPRA, ANPR A, ANPa, ANP A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHT-29 cells, HeLa cells, SGC-7901 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84043-1-RR targets NPR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A)
Calculated molecular weight 119 kDa
Observed molecular weight 119-125 kDa
GenBank accession numberNM_000906
Gene Symbol NPR1
Gene ID (NCBI) 4881
RRIDAB_3671610
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP16066
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NPR1(Atrial natriuretic peptide receptor 1), also named as NPRA , is a transmembrane protein expressed in the vascular endothelium. Activation of NPRA requires a binding of natriuretic peptides NPPA (ANP) or NPPB (BNP) to its extracellular domain and this promotes the production of cyclic guanosine monophosphate (cGMP), which in turn activates protein kinase cGMP‐dependent 1 (PRKG1 or commonly used as PKG).

Protocols

Product Specific Protocols
WB protocol for NPR1 antibody 84043-1-RRDownload protocol
IF protocol for NPR1 antibody 84043-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols