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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

NPR1 Polyclonal antibody

NPR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55116-1-AP

Synonyms

ANP A, ANPa, ANPR A, ANPRA, GC A, Guanylate cyclase A, GUC2A, GUCY2A, NPR1

Validation Data Gallery

Applications:
  • WB analysis of SGC-7901 using 55116-1-AP

    WB analysis of SGC-7901 using 55116-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 55116-1-AP (NPR1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 55116-1-AP

    IHC staining of human stomach cancer using 55116-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55116-1-AP (NPR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 55116-1-AP

    IHC staining of human stomach cancer using 55116-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55116-1-AP (NPR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inSGC-7901 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Sample-dependent, check data in validation data gallery

Product Information

55116-1-AP targets NPR1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A)
Calculated molecular weight 119 kDa
Observed molecular weight 119 kDa
GenBank accession numberNM_000906
Gene symbol NPR1
Gene ID (NCBI) 4881
RRIDAB_2881271
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NPR1 antibody 55116-1-APDownload protocol
IHC protocol for NPR1 antibody 55116-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

17β-Estradiol nongenomically induces vascular endothelial H2S release by promoting phosphorylation of cystathionine γ-lyase.

Authors - Xingyan Xu
View Article
humanWB

Int J Clin Exp Med

Impact of NPR-A expression in gastric cancer cells.

Authors - Jia Zhang
  • KD Validated
View Article