Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
30836-1-AP targets NPNT in WB, IF, ELISA applications and shows reactivity with Human, mouse , rat samples.
| Tested Reactivity | Human, mouse , rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34341 Product name: Recombinant human NPNT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 369-527 aa of BC166654 Sequence: TVDNRVQTDPQKPRGDVFIPRQPSNDLFEIFEIERGVSADDEAKDDPGVLVHSCNFDHGLCGWIREKDNDLHWEPIRDPAGGQYLTVSAAKAPGGKAARLVLPLGRLMHSGDLCLSFRHKVTGLHSGTLQVFVRKHGAHGAALWGRNGGHGWRQTQITL 相同性解析による交差性が予測される生物種 |
| Full Name | nephronectin |
| Calculated molecular weight | 62 kDa |
| Observed molecular weight | 62-70kDa |
| GenBank accession number | BC166654 |
| Gene Symbol | NPNT |
| Gene ID (NCBI) | 255743 |
| RRID | AB_3669763 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UXI9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NPNT antibody 30836-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
