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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NPM3 Recombinant antibody (85156-4-RR)

    WB analysis using 85156-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85156-4-RR (NPM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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NPM3 Recombinant antibody

NPM3 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85156-4-RR
Clone No.242863C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TMEM123, PORMIN, Nucleoplasmin-3, Nucleoplasmin 3, nucleophosmin/nucleoplasmin, 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HuH-7 cells, K-562 cells, Raji cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85156-4-RR targets NPM3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NPM3 fusion protein Ag2564 相同性解析による交差性が予測される生物種
Full Name nucleophosmin/nucleoplasmin, 3
Calculated molecular weight 178 aa, 19 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC054868
Gene Symbol NPM3
Gene ID (NCBI) 10360
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75607
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NPM3, a member of the nucleophosmin/ nucleoplasmin family, lacks intrinsic histone chaperone activity, inhibits histone assembly activity of NPM1 in vitro, and dramatically enhances transcription in a cellular system(PMID: 20073534). Human NPM3 is an abundant and widely expressed protein with primarily nuclear localization. The NPM3 protein migrated in SDS-PAGE gels as a doublet with apparent molecular masses of 25 and 27 kDa, which are larger than the 20.5 kDa mass predicted by the deduced amino acid sequence including the HA tag (PMID: 11722795). The NPM3 antibody can detect two bands with MW between 20-27 kDa.

Protocols

Product Specific Protocols
WB protocol for NPM3 antibody 85156-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols