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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NPM3 Polyclonal antibody

NPM3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11960-1-AP

Synonyms

NPM3, nucleophosmin/nucleoplasmin, 3, Nucleoplasmin 3, PORMIN, TMEM123

Validation Data Gallery

Applications:
  • WB analysis using 11960-1-AP

    WB analysis using 11960-1-AP

    Hela lysates were subjected to SDS PAGE followed by western blot with 11960-1-AP (NPM3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 11960-1-AP

    IP experiment of HeLa using 11960-1-AP

    IP result of anti-NPM3 (IP:11960-1-AP, 4ug; Detection:11960-1-AP 1:500) with HeLa cells lysate 3400ug.

  • IHC staining of human lymphoma using 11960-1-AP

    IHC staining of human lymphoma using 11960-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11960-1-AP (NPM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 11960-1-AP

    IHC staining of human lymphoma using 11960-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11960-1-AP (NPM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 11960-1-AP

    IF Staining of HeLa using 11960-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11960-1-AP (NPM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11960-1-AP targets NPM3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPM3 fusion protein Ag2564 相同性解析による交差性が予測される生物種
Full Name nucleophosmin/nucleoplasmin, 3
Calculated molecular weight 178 aa, 19 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC054868
Gene symbol NPM3
Gene ID (NCBI) 10360
RRIDAB_874199
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NPM3, a member of the nucleophosmin/ nucleoplasmin family, lacks intrinsic histone chaperone activity, inhibits histone assembly activity of NPM1 in vitro, and dramatically enhances transcription in a cellular system(PMID: 20073534). Human NPM3 is an abundant and widely expressed protein with primarily nuclear localization. The NPM3 protein migrated in SDS-PAGE gels as a doublet with apparent molecular masses of 25 and 27 kDa, which are larger than the 20.5 kDa mass predicted by the deduced amino acid sequence including the HA tag (PMID: 11722795). The NPM3 antibody can detect two bands with MW between 20-27 kDa.

Protocols

Product Specific Protocols
WB protocol for NPM3 antibody 11960-1-APDownload protocol
IHC protocol for NPM3 antibody 11960-1-APDownload protocol
IF protocol for NPM3 antibody 11960-1-APDownload protocol
IP protocol for NPM3 antibody 11960-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Cancer Lett

Pumilio1 regulates NPM3/NPM1 axis to promote PD-L1-mediated immune escape in gastric cancer

Authors - Han Wang
View Article