Validation Data Gallery
Tested Applications
| Positive WB detected in | 5637 cells, Caco-2 cells, PANC-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87535-4-RR targets NPLOC4 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag2219 Product name: Recombinant human NPLOC4 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 261-608 aa of BC025930 Sequence: DIPLGIRAEVAAIYEPPQIGTQNSLELLEDPKAEVVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRNKDTYFLSSEECITAGDFQNKHPNMCRLSPDGHFGSKFVTAVATGGPDNQVHFEGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDVDKFGNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAVRTRNEELAQTWKRSEQWATIEQLCSTVGGQLPGLHEYGAVGGSTHTATAAMWACQHCTFMNQPGTGHCEMCSLPRT 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear protein localization 4 homolog (S. cerevisiae) |
| Calculated molecular weight | 608 aa, 68 kDa |
| Observed molecular weight | 68-75 kDa |
| GenBank accession number | BC025930 |
| Gene Symbol | NPLOC4 |
| Gene ID (NCBI) | 55666 |
| RRID | AB_3745606 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8TAT6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NPLOC4 (NPL4 ubiquitin recognition factor) is an essential protein involved in numerous cellular processes, especially those related to the endoplasmic reticulum (ER) . It acts as a co-factor in the p97/VCP complex, which is vital for protein quality control and degradation via the ubiquitin-proteasome system. NPLOC4 plays a significant role in ER-associated degradation (ERAD), maintaining cellular homeostasis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NPLOC4 antibody 87535-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |