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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NPL Recombinant antibody (85608-3-RR)

    WB analysis using 85608-3-RR (same clone as 85608-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85608-3-RR (NPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85608-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using NPL Recombinant antibody (85608-3-RR)

    WB analysis of rat brain using 85608-3-RR (same clone as 85608-3-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 85608-3-RR (NPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85608-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85608-3-RR

    Affinity and Kinetic Characterization of 85608-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85608-3-RR against Human NPL were performed. The affinity constant is 4.26 nM.

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NPL Recombinant antibody, PBS Only

NPL Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85608-3-PBS
Clone No.243037E2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

c112, C1orf13, EC:4.1.3.3, N acetylneuraminate lyase, N-acetylneuraminate lyase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85608-3-PBS targets NPL in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NPL fusion protein Ag10137 相同性解析による交差性が予測される生物種
Full Name N-acetylneuraminate pyruvate lyase (dihydrodipicolinate synthase)
Calculated molecular weight 27 kDa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC058003
Gene Symbol NPL
Gene ID (NCBI) 80896
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BXD5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The N-acetylneuraminate lyase(NPL) from human (hNAL) which consists of 320 amino acids, has a molecular mass of 35.2 kDa and it catalyses the reversible aldol reaction of sialic acid, and has been extensively used in the synthesis of sialic acids and its analogues(PMID:19057931). The enzyme is widely distributed and are present in numerous pro- and eukaryotic cells, in which they are localized only in the cytosol(PMID:12453637). It belongs to the DHDPS family and has 5 isoforms produced by alternative splicing.