Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 22026-1-AP
mouse testis lysates were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 22026-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22026-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 22026-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 22026-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 22026-1-AP
IP result of anti-NPHP3 (IP:22026-1-AP, 4ug; Detection:22026-1-AP 1:1000) with mouse testis tissue lysate 4000ug.
IHC staining of human heart using 22026-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22026-1-AP (NPHP3 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 22026-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22026-1-AP (NPHP3 Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of hTERT-RPE1 cells using 22026-1-AP
IF result (the base and the lower half of cilia stain) of anti-NPHP3 (22026-1-AP, 1:50) with serum-starved hTERT-RPE1 (MeOH fixed) by Dr. Moshe Kim.
IF Staining of MDCK using 22026-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 22026-1-AP (NPHP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

NPHP3 Polyclonal antibody

NPHP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22026-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Nephrocystin-3, Nephrocystin 3, MKS7, KIAA2000

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse testis tissue, HepG2 cells, HEK-293 cells, mouse liver tissue, human testis tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	hTERT-RPE1 cells, MDCK cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below
IF	See 5 publications below

Product Information

22026-1-AP targets NPHP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity	human, mouse, rat, canine
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16888 Product name: Recombinant human NPHP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-131 aa of BC068082 Sequence: MGTASSLVSPAGGEVIEDTYGAGGGEACEIPVEVKPKARLLRNSFRRGAGAAAGAGPGSLPRGVGAGGLLGASFKSTGSSVPELEYAAAEYERLRKEYEIFRVSKNQELLSMGRREAKLDTENKRLRAELQ 相同性解析による交差性が予測される生物種
Full Name	nephronophthisis 3 (adolescent)
Calculated molecular weight	1330 aa, 151 kDa
Observed molecular weight	145-151 kDa
GenBank accession number	BC068082
Gene Symbol	NPHP3
Gene ID (NCBI)	27031
RRID	AB_2878975
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q7Z494
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for NPHP3 antibody 22026-1-AP	Download protocol
IHC protocol for NPHP3 antibody 22026-1-AP	Download protocol
IP protocol for NPHP3 antibody 22026-1-AP	Download protocol
WB protocol for NPHP3 antibody 22026-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nat Methods Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia. Authors - Su Steven S View Article
human	IF	Am J Hum Genet ARL3 Mutations Cause Joubert Syndrome by Disrupting Ciliary Protein Composition. Authors - Sumaya Alkanderi View Article
human	IF	Hum Mol Genet Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelial cells. Authors - Nele Schwarz View Article
mouse	IF	Int J Mol Sci Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses. Authors - Shweta Suiwal View Article
mouse	IF	Mol Biol Cell Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia Authors - Vivek Reddy Palicharla View Article
		Methods Cell Biol Single-molecule imaging in the primary cilium Authors - Lucien E Weiss View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 22026-1-AP

WB analysis of mouse liver using 22026-1-AP

WB analysis of HEK-293 using 22026-1-AP

WB analysis of human testis using 22026-1-AP

WB analysis of HepG2 using 22026-1-AP

IP experiment of mouse testis using 22026-1-AP

IHC staining of human heart using 22026-1-AP

IHC staining of human heart using 22026-1-AP

IF Staining of hTERT-RPE1 cells using 22026-1-AP

IF Staining of MDCK using 22026-1-AP

NPHP3 Polyclonal antibody

NPHP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.

ARL3 Mutations Cause Joubert Syndrome by Disrupting Ciliary Protein Composition.

Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelial cells.

Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses.

Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia

Single-molecule imaging in the primary cilium