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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NPFFR2 Polyclonal antibody

NPFFR2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 19505-1-AP

Synonyms

G protein coupled receptor 74, GPR74, HLWAR77, neuropeptide FF receptor 2, NPFFR2

Validation Data Gallery

Applications:
  • IHC staining of human small intestine using 19505-1-AP

    IHC staining of human small intestine using 19505-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19505-1-AP (NPFFR2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human small intestine using 19505-1-AP

    IHC staining of human small intestine using 19505-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19505-1-AP (NPFFR2 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 19505-1-AP

    IHC staining of human testis using 19505-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19505-1-AP (NPFFR2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 19505-1-AP

    IHC staining of human testis using 19505-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19505-1-AP (NPFFR2 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human placenta using 19505-1-AP

    IHC staining of human placenta using 19505-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19505-1-AP (NPFFR2 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 19505-1-AP

    IHC staining of human placenta using 19505-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19505-1-AP (NPFFR2 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inhuman small intestine tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

19505-1-AP targets NPFFR2 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name neuropeptide FF receptor 2
Calculated molecular weight 60 kDa
GenBank accession numberNM_053036
Gene symbol NPFFR2
Gene ID (NCBI) 10886
RRIDAB_2878584
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NPFFR2, also named as GPR74, NPFF2 and NPGPR, belongs to the G-protein coupled receptor 1 family. NPFFR2 is a receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides which also known as morphine-modulating peptides. NPFFr2 can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. NPFFR2 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The antibody can recognize isoform1, isoform2 and isoform3 of NPFFR2.

Protocols

Product Specific Protocols
IHC protocol for NPFFR2 antibody 19505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols