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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NPEPPS Recombinant antibody (84230-4-RR)

    WB analysis using 84230-4-RR (same clone as 84230-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84230-4-RR (NPEPPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84230-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using NPEPPS Recombinant antibody (84230-4-RR)

    IHC staining of mouse liver using 84230-4-RR (same clone as 84230-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84230-4-RR (NPEPPS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84230-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84230-4-RR

    Affinity and Kinetic Characterization of 84230-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84230-4-RR against Human NPEPPS were performed. The affinity constant is 0.956 nM.

NPEPPS Recombinant monoclonal antibody, PBS Only

NPEPPS Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84230-4-PBS
Clone No.241502G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

PSA, MP100, AAP S, 241502G5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84230-4-PBS targets NPEPPS in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag36035

Product name: Recombinant human NPEPPS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 711-919 aa of BC065294

Sequence: GKLGKAGHKATLEEARRRFKDHVEGKQILSADLRSPVYLTVLKHGDGTTLDIMLKLHKQADMQEEKNRIERVLGATLLPDLIQKVLTFALSEEVRPQDTVSVIGGVAGGSKHGRKAAWKFIKDNWEELYNRYQGGFLISRLIKLSVEGFAVDKMAGEVKAFFESHPAPSAERTIQQCCENILLNAAWLKRDAESIHQYLLQRKASPPTV

相同性解析による交差性が予測される生物種
Full Name aminopeptidase puromycin sensitive
Observed molecular weight103 kDa
GenBank accession numberBC065294
Gene Symbol NPEPPS
Gene ID (NCBI) 9520
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP55786
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NPEPPS, also known as PSA, is a member of the oxytocinase subfamily of the M1 aminopeptidase family. NPEPPS encodes puromycin-sensitive aminopeptidase, a zinc metallopeptidase that hydrolyzes amino acids from the N-terminus of its substrate. The protein has 2 isoforms and is located in the cytoplasm.

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