Validation Data Gallery
Tested Applications
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24162-1-AP targets NPBWR2 in IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20874 Product name: Recombinant human NPBWR2 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-211 aa of BC067481 Sequence: MQAAGHPEPLDSRGSFSLPTMGANVSQDNGTGHNATFSEPLPFLYVLLPAVYSGICAVGLTGNTAVILVILRAPKMKTVTNVFILNLAVADGLFTLVLPVNIAEHLLQYWPFGELLCKLVLAVDHYNIFSSIYFLAVMSVDRYLVVLATVRSRHMPWRTYRGAKVASLCVWLGVTVLVLPFFSFAGVYSNELQVPSCGLSFPWPEQVWFKA 相同性解析による交差性が予測される生物種 |
| Full Name | neuropeptides B/W receptor 2 |
| Calculated molecular weight | 333 aa, 37 kDa |
| GenBank accession number | BC067481 |
| Gene Symbol | NPBWR2 |
| Gene ID (NCBI) | 2832 |
| RRID | AB_2879442 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P48146 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NPBWR1 (GPR7) and NPBWR2 (GPR8) are two structurally related G-protein coupled receptors. These two receptors share high sequence identity to each other and significant similarity with opioid and somatostatin receptors (PMID: 10407191). Neuropeptide B (NPB) and neuropeptide W (NPW) are endogenous peptide ligands of these receptors. NPBWR1 was found highly conserved in both human and rodents while NPBWR2 gene was not found in the rodent genomes (PMID: 7590751; 10407191). NPBWR1 and NPBWR2 are implicated in energy homeostasis, pain, and emotion (PMID: 23515889).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NPBWR2 antibody 24162-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
