Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human brain tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25517-1-AP targets NPB in IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21877 Product name: Recombinant human NPB protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 53-125 aa of BC126128 Sequence: ARRSQPYRGAEPPGGAGASPELQLHPRLRSLAVCVQDVAPNLQRCERLPDGRGTYQCKANVFLSLRAADCLAA 相同性解析による交差性が予測される生物種 |
| Full Name | neuropeptide B |
| Calculated molecular weight | 125 aa, 13 kDa |
| Observed molecular weight | ~10 kDa |
| GenBank accession number | BC126128 |
| Gene Symbol | NPB |
| Gene ID (NCBI) | 256933 |
| RRID | AB_2880114 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NG41 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Neuropeptide B, also named as NPB, PPL7 and PPNBP, can be cleaved into two chains, Neuropeptide B-23 (NPB23, hL7) and Neuropeptide B-29 (NPB29, hL7C). It is involved in the regulation of feeding, neuroendocrine system, memory, learning and in the afferent pain pathway. It is widely expressed in central nervous system.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NPB antibody 25517-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |