Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, MCF-7 cells, HEK-293 cells, PC-12 cells, mouse kidney tissue, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
30131-1-AP targets NOX3 in WB, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32601 Product name: Recombinant human NOX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 223-395 aa of NM_015718 Sequence: GRIVRGQTQDSLSLHNITFCRDRYAEWQTVAQCPVPQFSGKEPSAWKWILGPVVLYACERIIRFWRFQQEVVITKVVSHPSGVLELHMKKRGFKMAPGQYILVQCPAISSLEWHPFTLTSAPQEDFFSVHIRAAGDWTAALLEAFGAEGQALQEPWSLPRLAVDGPFGTALTD 相同性解析による交差性が予測される生物種 |
| Full Name | NADPH oxidase 3 |
| Calculated molecular weight | 64kd |
| Observed molecular weight | 65 kDa |
| GenBank accession number | NM_015718 |
| Gene Symbol | NOX3 |
| Gene ID (NCBI) | 50508 |
| RRID | AB_2935517 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HBY0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NOX3 is a member of NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. NOX3 plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. The antibody is specific to NOX3.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NOX3 antibody 30131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
