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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using NOVA2 Polyclonal antibody (55002-1-AP)

    WB analysis of mouse brain using 55002-1-AP (same clone as 55002-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55002-1-AP (NOVA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55002-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NOVA2 Polyclonal antibody (55002-1-AP)

    WB analysis using 55002-1-AP (same clone as 55002-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55002-1-AP (NOVA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55002-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NOVA2 Polyclonal antibody (55002-1-AP)

    IP experiment of mouse brain using 55002-1-AP (same clone as 55002-1-PBS)

    IP result of anti-NOVA2 (IP:55002-1-AP, 4ug; Detection:55002-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 55002-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NOVA2 Polyclonal antibody, PBS Only

NOVA2 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 55002-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55002-1-PBS targets NOVA2 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name neuro-oncological ventral antigen 2
Calculated molecular weight 49 kDa
Observed molecular weight50-55 kDa, 65-70 kDa
GenBank accession numberNM_002516
Gene Symbol NOVA2
Gene ID (NCBI) 4858
RRIDAB_10858926
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UNW9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NOVA2 also named as ANOVA and NOVA3. It regulates RNA splicing or metabolism in a specific subset of developing neurons. NOVA2 binds single strand RNA. The antibody is specific to NOVA2. By the alternative splicing, NOVA2 can be detected as 50-55 and 70-80 kDa (PMID:26446569, PMID:9789075).

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