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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOTCH4 Polyclonal antibody (33280-1-AP)

    WB analysis using 33280-1-AP (same clone as 33280-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33280-1-AP (NOTCH4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33280-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using NOTCH4 Polyclonal antibody (33280-1-AP)

    IHC staining of mouse lung using 33280-1-AP (same clone as 33280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 33280-1-AP (NOTCH4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33280-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using NOTCH4 Polyclonal antibody (33280-1-AP)

    IHC staining of mouse lung using 33280-1-AP (same clone as 33280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 33280-1-AP (NOTCH4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33280-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NOTCH4 Polyclonal antibody (33280-1-AP)

    IHC staining of mouse heart using 33280-1-AP (same clone as 33280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 33280-1-AP (NOTCH4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33280-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NOTCH4 Polyclonal antibody (33280-1-AP)

    IHC staining of mouse heart using 33280-1-AP (same clone as 33280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 33280-1-AP (NOTCH4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33280-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NOTCH4 Polyclonal antibody (33280-1-AP)

    IHC staining of mouse kidney using 33280-1-AP (same clone as 33280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 33280-1-AP (NOTCH4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33280-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using NOTCH4 Polyclonal antibody (33280-1-AP)

    IHC staining of mouse kidney using 33280-1-AP (same clone as 33280-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 33280-1-AP (NOTCH4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33280-1-PBS in a different storage buffer formulation.

NOTCH4 Polyclonal antibody, PBS Only

NOTCH4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 33280-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

hNotch4, INT3, Notch 4, Notch 4 extracellular truncation, Notch 4 intracellular domain

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

33280-1-PBS targets NOTCH4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag36738

Product name: Recombinant human NOTCH4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 309-648 aa of BC140782

Sequence: DCSEDVDECETQGPPHCRNGGTCQNSAGSFHCVCVSGWGGTSCEENLDDCIAATCAPGSTCIDRVGSFSCLCPPGRTGLLCHLEDMCLSQPCHGDAQCSTNPLTGSTLCLCQPGYSGPTCHQDLDECLMAQQGPSPCEHGGSCLNTPGSFNCLCPPGYTGSRCEADHNECLSQPCHPGSTCLDLLATFHCLCPPGLEGQLCEVETNECASAPCLNHADCHDLLNGFQCICLPGFSGTRCEEDIDECRSSPCANGGQCQDQPGAFHCKCLPGFEGPRCQTEVDECLSDPCPVGASCLDLPGAFFCLCPSGFTGQLCEVPLCAPNLCQPKQICKDQKDKANC

相同性解析による交差性が予測される生物種
Full Name Notch homolog 4 (Drosophila)
Calculated molecular weight 2003 aa, 210 kDa
Observed molecular weight210 kDa
GenBank accession numberBC140782
Gene Symbol NOTCH4
Gene ID (NCBI) 4855
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ99466
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Neurogenic locus notch homolog protein 4 (NOTCH4) is a receptor involved in the Notch signaling pathway, which is synthesized as a precursor that undergoes proteolytic cleavage to form an active receptor. NOTCH4 interacts with various ligands, leading to the release of the Notch intracellular domain (NICD), which translocates to the nucleus and regulates gene expression (PMID: 28794168; 19379690; 17148788). NOTCH4 is involved in various biological processes, including embryonic development, immune cell differentiation, and tumorigenesis (PMID: 24667410; 34925319).

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