Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 55114-1-AP (same clone as 55114-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.
WB analysis using 55114-1-AP (same clone as 55114-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 55114-1-AP (same clone as 55114-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.
WB analysis of U2OS using 55114-1-AP (same clone as 55114-1-PBS)
U2OS cells were subjected to SDS PAGE followed by western blot with 55114-1-AP (NOTCH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 55114-1-AP (same clone as 55114-1-PBS)
IP result of anti-NOTCH3 (IP:55114-1-AP, 4ug; Detection:55114-1-AP 1:800) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 55114-1-AP (same clone as 55114-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 55114-1-AP (same clone as 55114-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 55114-1-AP (same clone as 55114-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 55114-1-AP (same clone as 55114-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 55114-1-AP (same clone as 55114-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 55114-1-AP (same clone as 55114-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55114-1-AP (NOTCH3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 55114-1-AP (same clone as 55114-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NOTCH3 antibody (55114-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 55114-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

NOTCH3 Polyclonal antibody, PBS Only

NOTCH3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 55114-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CADASIL, CASIL, Notch 3, Notch homolog 3 (Drosophila)

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55114-1-PBS targets NOTCH3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	Notch homolog 3 (Drosophila)
Calculated molecular weight	244 kDa
Observed molecular weight	250-260 kDa, 100 kDa
GenBank accession number	NM_000435
Gene Symbol	NOTCH3
Gene ID (NCBI)	4854
RRID	AB_10858393
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UM47
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

NOTCH3 belongs to the NOTCH family. It functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. NOTCH3 affects the implementation of differentiation, proliferation and apoptotic programs. Defects in NOTCH3 are the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). The antibody is specific to NOTCH3. Notch3 is a single-pass type I membrane protein exposed at the cell surface as a heterodimer of 2321 amino acids. It is synthesized as a precursor with a molecular weight of ~280 kDa (Notch3 full-length), which is cleaved (S1 cleavage) into a 210 kDa extracellular fragment and a 97 kDa intracellular fragment (PMID: 21702048).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 55114-1-AP (same clone as 55114-1-PBS)

WB analysis using 55114-1-AP (same clone as 55114-1-PBS)

WB analysis of COLO 320 using 55114-1-AP (same clone as 55114-1-PBS)

WB analysis of U2OS using 55114-1-AP (same clone as 55114-1-PBS)

IP experiment of HeLa using 55114-1-AP (same clone as 55114-1-PBS)

IHC staining of human lung cancer using 55114-1-AP (same clone as 55114-1-PBS)

IHC staining of human lung cancer using 55114-1-AP (same clone as 55114-1-PBS)

IHC staining of human breast cancer using 55114-1-AP (same clone as 55114-1-PBS)

IHC staining of human breast cancer using 55114-1-AP (same clone as 55114-1-PBS)

IHC staining of human kidney using 55114-1-AP (same clone as 55114-1-PBS)