Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICC

WB analysis using 20116-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 20116-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 20116-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 20116-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20116-1-AP (NOSTRIN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of human placenta using 20116-1-AP
IP result of anti-NOSTRIN (IP:20116-1-AP, 4ug; Detection:20116-1-AP 1:500) with human placenta tissue lysate 1600ug.
IHC staining of human heart using 20116-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20116-1-AP (NOSTRIN Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human heart using 20116-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20116-1-AP (NOSTRIN Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of mouse testis using 20116-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 20116-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 20116-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 20116-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20116-1-AP (NOSTRIN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse kidney using 20116-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NOSTRIN antibody (20116-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse kidney using 20116-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using NOSTRIN antibody (20116-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 20116-1-AP
Immunofluorescent analysis of HepG2 cells using 20116-1-AP (NOSTRIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

NOSTRIN Polyclonal antibody

NOSTRIN Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 20116-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

eNOS-trafficking inducer, eNOS trafficking inducer, DaIP2, BM247 homolog

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse heart tissue, human placenta tissue, mouse liver tissue, mouse lung tissue
Positive IP detected in	human placenta tissue
Positive IHC detected in	human heart tissue, mouse testis tissue, human hepatocirrhosis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse kidney tissue
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

20116-1-AP targets NOSTRIN in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13989 Product name: Recombinant human NOSTRIN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 122-428 aa of BC014189 Sequence: KWENTLENCYQSILELEKERIQLLCNNLNQYSQHISLFGQTLTTCHTQIHCAISKIDIEKDIQAVMEETAILSTENKSEFLLTDYFEEDPNSAMDKERRKSLLKPKLLRLQRDIEKASKDKEGLERMLKTYSSTSSFSDAKSQKDTAALMDENNLKLDLLEANSYKLSSMLAELEQRPQPSHPCSNSIFRWREKEHTHSYVKISRPFLMKRLENIVSKASSGGQSNPGSSTPAPGAAQLSSRLCKALYSFQARQDDELNLEKGDIVIIHEKKEEGWWFGSLNGKKGHFPAAYVEELPSNAGNTATKA 相同性解析による交差性が予測される生物種
Full Name	nitric oxide synthase trafficker
Calculated molecular weight	506 aa, 58 kDa
Observed molecular weight	64 kDa
GenBank accession number	BC014189
Gene Symbol	NOSTRIN
Gene ID (NCBI)	115677
RRID	AB_10666167
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IVI9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NOSTRIN is classically known as an endothelial cell protein that sequesters eNOS and inhibits NO production. NOSTRIN contains a single polypeptide chain of 506-aa residues of 58 kDa with an N-terminal cdc15 domain and a C-terminal SH3 domain. NOSTRIN mRNA is abundant in highly vascularized tissues such as placenta, kidney, lung, and heart, and NOSTRIN protein is expressed in vascular endothelial cells.

Protocols

Product Specific Protocols
IF protocol for NOSTRIN antibody 20116-1-AP	Download protocol
IHC protocol for NOSTRIN antibody 20116-1-AP	Download protocol
IP protocol for NOSTRIN antibody 20116-1-AP	Download protocol
WB protocol for NOSTRIN antibody 20116-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Redox Biol Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro. Authors - Dejuan Li View Article
rat	WB	J Inflamm Res TNF-α and IL-1β Promote Renal Podocyte Injury in T2DM Rats by Decreasing Glomerular VEGF/eNOS Expression Levels and Altering Hemodynamic Parameters Authors - Jufang Wang View Article
		Naunyn Schmiedebergs Arch Pharmacol Thymoquinone mitigate ischemia-reperfusion-induced liver injury in rats: a pivotal role of nitric oxide signaling pathway. Authors - Mohamed Abd-Elbaset View Article
rat	WB	Mol Med Rep Appropriate timing for hypothermic machine perfusion to preserve livers donated after circulatory death. Authors - Xiaoyan Hu View Article
rat	WB	Mol Med Rep UBIAD1 expression is associated with cardiac hypertrophy in spontaneously hypertensive rats. Authors - Bingju Yan View Article
human	WB	Mol Med Rep Depletion of ubiA prenyltransferase domain containing 1 expression promotes angiotensin II‑induced hypertrophic response in AC16 human myocardial cells via modulating the expression levels of coenzyme Q10 and endothelial nitric oxide synthase. Authors - Bingju Yan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 20116-1-AP

WB analysis of K-562 using 20116-1-AP

WB analysis of human placenta using 20116-1-AP

WB analysis using 20116-1-AP

IP experiment of human placenta using 20116-1-AP

IHC staining of human heart using 20116-1-AP

IHC staining of human heart using 20116-1-AP

IHC staining of mouse testis using 20116-1-AP

IHC staining of mouse testis using 20116-1-AP

IHC staining of human hepatocirrhosis using 20116-1-AP

IHC staining of human hepatocirrhosis using 20116-1-AP

IF Staining of mouse kidney using 20116-1-AP

IF Staining of mouse kidney using 20116-1-AP

IF Staining of HepG2 using 20116-1-AP

NOSTRIN Polyclonal antibody

NOSTRIN Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cardioprotection of CAPE-oNO2 against myocardial ischemia/reperfusion induced ROS generation via regulating the SIRT1/eNOS/NF-κB pathway in vivo and in vitro.

TNF-α and IL-1β Promote Renal Podocyte Injury in T2DM Rats by Decreasing Glomerular VEGF/eNOS Expression Levels and Altering Hemodynamic Parameters

Thymoquinone mitigate ischemia-reperfusion-induced liver injury in rats: a pivotal role of nitric oxide signaling pathway.

Appropriate timing for hypothermic machine perfusion to preserve livers donated after circulatory death.

UBIAD1 expression is associated with cardiac hypertrophy in spontaneously hypertensive rats.

Depletion of ubiA prenyltransferase domain containing 1 expression promotes angiotensin II‑induced hypertrophic response in AC16 human myocardial cells via modulating the expression levels of coenzyme Q10 and endothelial nitric oxide synthase.