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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOSIP Polyclonal antibody (27979-1-AP)

    WB analysis using 27979-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NOSIP Polyclonal antibody (27979-1-AP)

    WB analysis using 27979-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using NOSIP Polyclonal antibody (27979-1-AP)

    WB analysis of A431 using 27979-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 27979-1-AP (NOSIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using NOSIP Polyclonal antibody (27979-1-AP)

    IHC staining of human colon using 27979-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using NOSIP Polyclonal antibody (27979-1-AP)

    IHC staining of human colon using 27979-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using NOSIP Polyclonal antibody (27979-1-AP)

    IHC staining of human placenta using 27979-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using NOSIP Polyclonal antibody (27979-1-AP)

    IHC staining of mouse pancreas using 27979-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using NOSIP Polyclonal antibody (27979-1-AP)

    IHC staining of rat kidney using 27979-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using NOSIP Polyclonal antibody (27979-1-AP)

    IHC staining of rat pancreas using 27979-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using NOSIP Polyclonal antibody (27979-1-AP)

    IHC staining of human lung using 27979-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using NOSIP Polyclonal antibody (27979-1-AP)

    IHC staining of human lung using 27979-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27979-1-AP (NOSIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NOSIP Polyclonal antibody (27979-1-AP)

    IF Staining of HeLa using 27979-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 27979-1-AP (NOSIP antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

NOSIP Polyclonal antibody

NOSIP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27979-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CGI 25, CGI-25, E3 ubiquitin-protein ligase NOSIP, EC:2.3.2.27, eNOS interacting protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, A431 cells, A549 cells, Ramos cells, HepG2 cells, L02 cells
Positive IHC detected inhuman colon tissue, human lung tissue, rat pancreas tissue, mouse pancreas tissue, rat kidney tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27979-1-AP targets NOSIP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27569

Product name: Recombinant human NOSIP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-301 aa of BC010077

Sequence: MTRHGKNCTAGAVYTYHEKKKDTAASGYGTQNIRLSRDAVKDFDCCCLSLQPCHDPVVTPDGYLYEREAILEYILHQKKEIARQMKAYEKQRGTRREEQKELQRAASQDHVRGFLEKESAIVSRPLNPFTAKALSGTSPDDVQPGPSVGPPSKDKDKVLPSFWIPSLTPEAKATKLEKPSRTVTCPMSGKPLRMSDLTPVHFTPLDSSVDRVGLITRSERYVCAVTRDSLSNATPCAVLRPSGAVVTLECVEKLIRKDMVDPVTGDKLTDRDIIVLQRGGTGFAGSGVKLQAEKSRPVMQA

相同性解析による交差性が予測される生物種
Full Name nitric oxide synthase interacting protein
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC010077
Gene Symbol NOSIP
Gene ID (NCBI) 51070
RRIDAB_2881027
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y314
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for NOSIP antibody 27979-1-APDownload protocol
IHC protocol for NOSIP antibody 27979-1-APDownload protocol
WB protocol for NOSIP antibody 27979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Selective identification of epigenetic regulators at methylated genomic sites by SelectID

Authors - Wenchang Qian
View Article

Sci Rep

NOSIP promotes cell proliferation and motility by targeting SPTAN1 for ubiquitination and degradation in clear cell renal cell carcinoma.

Authors - Ruoxue Cao
View Article
humanWB,IHC

iScience

Nosip is a potential therapeutic target in hepatocellular carcinoma cells

Authors - Junjie Gao
  • KD Validated
View Article
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