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Validation Data Gallery

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  • Western Blot (WB) analysis of bEnd.3 cells using NOS3 Recombinant antibody (85020-4-RR)

    WB analysis of bEnd.3 using 85020-4-RR

    bEnd.3 cells were subjected to SDS PAGE followed by western blot with 85020-4-RR (NOS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HUVEC cells using NOS3 Recombinant antibody (85020-4-RR)

    WB analysis of HUVEC using 85020-4-RR

    HUVEC cells were subjected to SDS PAGE followed by western blot with 85020-4-RR (NOS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85020-4-RR

    Affinity and Kinetic Characterization of 85020-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85020-4-RR against Human NOS3 were performed. The affinity constant is 3.17 nM.

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NOS3 Recombinant antibody

NOS3 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85020-4-RR
Clone No.242543E10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

eNOS, EC-NOS, ECNOS, EC:1.14.13.39, cNOS

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inbEnd.3 cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85020-4-RR targets NOS3 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NOS3 fusion protein Ag25712 相同性解析による交差性が予測される生物種
Full Name nitric oxide synthase 3 (endothelial cell)
Observed molecular weight140 kDa
Gene Symbol eNOS
Gene ID (NCBI) 4846
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29474
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Endothelial NOS(eNOS), also known as nitric oxide synthase 3(NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. NOS3 has 3 isoforms of 68, 69, 133 kDa. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine.

Protocols

Product Specific Protocols
WB protocol for NOS3 antibody 85020-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols