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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOP58 Recombinant monoclonal antibody (82879-1-RR)

    WB analysis using 82879-1-RR (same clone as 82879-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82879-1-RR (NOP58 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using NOP58 Recombinant monoclonal antibody (82879-1-RR)

    WB analysis of MCF-7 using 82879-1-RR (same clone as 82879-1-PBS)

    WB result of NOP58 antibody (82879-1-RR; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NOP58 transfected MCF-7 cells. This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using NOP58 Recombinant monoclonal antibody (82879-1-RR)

    IP experiment of HeLa using 82879-1-RR (same clone as 82879-1-PBS)

    IP result of anti-NOP58 (IP:82879-1-RR, 4ug; Detection:82879-1-RR 1:2000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NOP58 Recombinant monoclonal antibody (82879-1-RR)

    IHC staining of human prostate cancer using 82879-1-RR (same clone as 82879-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82879-1-RR (NOP58 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NOP58 Recombinant monoclonal antibody (82879-1-RR)

    IHC staining of human prostate cancer using 82879-1-RR (same clone as 82879-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82879-1-RR (NOP58 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NOP58 Recombinant monoclonal antibody (82879-1-RR)

    IF Staining of U2OS using 82879-1-RR (same clone as 82879-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NOP58 antibody (82879-1-RR) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using NOP58 Recombinant monoclonal antibody (82879-1-RR)

    FC experiment of HeLa using 82879-1-RR (same clone as 82879-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NOP58 Recombinant antibody (82879-1-RR, Clone:230058C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82879-1-RR

    Affinity and Kinetic Characterization of 82879-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82879-1-RR against Human NOP58 were performed. The affinity constant is 5.53 nM.

  • Affinity and Kinetic Characterization of 82879-1-RR

    Affinity and Kinetic Characterization of 82879-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82879-1-RR against Human NOP58 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

NOP58 Recombinant monoclonal antibody, PBS Only

NOP58 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 82879-1-PBS
Clone No.230058C7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

230058C7, HSPC120, NOL5, NOP5, NOP5/NOP58

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82879-1-PBS targets NOP58 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5740

Product name: Recombinant human NOP58 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 279-529 aa of BC032592

Sequence: MMAIAPNVTVMVGELVGARLIAHAGSLLNLAKHAASTVQILGAEKALFRALKSRRDTPKYGLIYHASLVGQTSPKHKGKISRMLAAKTVLAIRYDAFGEDSSSAMGVENRAKLEARLRTLEDRGIRKISGTGKALAKTEKYEHKSEVKTYDPSGDSTLPTCSKKRKIEQVDKEDEITEKKAKKAKIKVKVEEEEEEKVAEEEETSVKKKKKRGKKKHIKEEPLSEEEPCTSTAIASPEKKKKKKKKRENED

相同性解析による交差性が予測される生物種
Full Name NOP58 ribonucleoprotein homolog (yeast)
Calculated molecular weight 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC032592
Gene Symbol NOP58
Gene ID (NCBI) 51602
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2X3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The box C/D snoRNPs are named for the short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. The box C/D sequences are also required for multiple aspects. of snoRNA biogenesis, including RNA stability, intronic processing, nucleolar targeting, nuclear retention, and 59 trimethylguanosine cap formation (PMID:9649444,8878486). Nop58 is a component common to the box C/D small nucleolar ribonucleoprotein (PMID:10606270).

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