Validation Data Gallery

Filter: AllWBIF/ICC

WB analysis of MDA-MB-453s using 26854-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 26854-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 26854-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 26854-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 26854-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 26854-1-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 26854-1-AP (NOP14 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 26854-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 26854-1-AP (NOP14 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

NOP14 Polyclonal antibody

NOP14 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 26854-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C4orf9, NOL14, Nucleolar protein 14, RES4 25, RES425

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MDA-MB-453s cells, BxPC-3 cells, MCF-7 cells, mouse lung tissue, rat kidney tissue
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 3 publications below
IF	See 1 publications below

Product Information

26854-1-AP targets NOP14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25476 Product name: Recombinant human NOP14 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-95 aa of BC026035 Sequence: MAKAKKVGARRKASGAPAGARGGPAKANSNPFEVKVNRQKFQILGRKTRHDVGLPGVSRARALRKRTQTLLKEYKERDKSNVFRDKRFGEYNSNM 相同性解析による交差性が予測される生物種
Full Name	NOP14 nucleolar protein homolog (yeast)
Calculated molecular weight	98 kDa
Observed molecular weight	98 kDa
GenBank accession number	BC026035
Gene Symbol	NOP14
Gene ID (NCBI)	8602
RRID	AB_2880657
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P78316
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for NOP14 antibody 26854-1-AP	Download protocol
IF protocol for NOP14 antibody 26854-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB,IHC	Cell Prolif CCND1, NOP14 and DNMT3B are involved in miR-502-5p-mediated inhibition of cell migration and proliferation in bladder cancer. Authors - Yufan Ying KD Validated View Article
human	WB,IHC,IF	Aging (Albany NY) Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression Authors - Guoyang Zhang View Article
human	WB	Int J Biol Macromol CircNOP14 increases the radiosensitivity of hepatocellular carcinoma via inhibition of Ku70-dependent DNA damage repair Authors - Letao Lin View Article
human	WB,IHC	Front Biosci (Landmark Ed) NOP14 as a Potential Predictor of Adult-Type Diffuse Glioma Prognosis and Immunotherapy, is Related to Cell Migration, Proliferation, and CD8+T Cell Infiltration Authors - Qiu-Si Tian View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MDA-MB-453s using 26854-1-AP

WB analysis of BxPC-3 using 26854-1-AP

WB analysis of MCF-7 using 26854-1-AP

WB analysis of mouse lung using 26854-1-AP

WB analysis of rat kidney using 26854-1-AP

WB analysis of BxPC-3 using 26854-1-AP

IF Staining of HepG2 using 26854-1-AP

NOP14 Polyclonal antibody

NOP14 Polyclonal Antibody for WB, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

CCND1, NOP14 and DNMT3B are involved in miR-502-5p-mediated inhibition of cell migration and proliferation in bladder cancer.

Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression

CircNOP14 increases the radiosensitivity of hepatocellular carcinoma via inhibition of Ku70-dependent DNA damage repair

NOP14 as a Potential Predictor of Adult-Type Diffuse Glioma Prognosis and Immunotherapy, is Related to Cell Migration, Proliferation, and CD8+T Cell Infiltration