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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOP10 Polyclonal antibody (22769-1-AP)

    WB analysis using 22769-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22769-1-AP (NOP10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NOP10 Polyclonal antibody (22769-1-AP)

    IHC staining of human pancreas cancer using 22769-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22769-1-AP (NOP10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NOP10 Polyclonal antibody (22769-1-AP)

    IHC staining of human pancreas cancer using 22769-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22769-1-AP (NOP10 antibody) at dilution of 1:400 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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NOP10 Polyclonal antibody

NOP10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22769-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

NOP10P, NOP 10, NOLA3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22769-1-AP targets NOP10 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOP10 fusion protein Ag18718 相同性解析による交差性が予測される生物種
Full Name NOP10 ribonucleoprotein homolog (yeast)
Calculated molecular weight 64 aa, 77 kDa
Observed molecular weight10-12 kDa
GenBank accession numberBC063023
Gene Symbol NOP10
Gene ID (NCBI) 55505
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9NPE3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleolar protein 10 (NOP10) is a component of the H/ACA small nucleolar ribonucleoprotein (snoRNP) complex and is essential for maintaining telomerase activity and RNA pseudouridylation. NOP10 is abnormally expressed in a variety of cancers such as breast cancer, pancreatic cancer, lung cancer, etc., and high expression is usually associated with poor prognosis(PMID: 33161513; 39538226).

Protocols

Product Specific Protocols
WB protocol for NOP10 antibody 22769-1-APDownload protocol
IHC protocol for NOP10 antibody 22769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols