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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of HEK-293 using 16083-1-AP (same clone as 16083-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of HEK-293 using 16083-1-AP (same clone as 16083-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of PC-3 using 16083-1-AP (same clone as 16083-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of PC-3 using 16083-1-AP (same clone as 16083-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of HepG2 using 16083-1-AP (same clone as 16083-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of HepG2 using 16083-1-AP (same clone as 16083-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of PC-3 using 16083-1-AP (same clone as 16083-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of HEK-293 using 16083-1-AP (same clone as 16083-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using NOL9 Polyclonal antibody (16083-1-AP)

    WB analysis of PC-3 using 16083-1-AP (same clone as 16083-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16083-1-AP (NOL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16083-1-PBS in a different storage buffer formulation.

NOL9 Polyclonal antibody, PBS Only

NOL9 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 16083-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16083-1-PBS targets NOL9 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9082

Product name: Recombinant human NOL9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 82-430 aa of BC009257

Sequence: TEPVLGPPFTHLRTPQKMVYYGKPSCKNNYENYIDIVKYVFSAYKRESPLIVNTMGWVSDQGLLLLIDLIRLLSPSHVVQFRSDHSKYMPDLTPQYVDDMDGLYTKSKTKMRNRRFRLAAFADALEFADEEKESPVEFTGHKLIGVYTDFAFRITPRNRESHNKILRDLSILSYLSQLQPPMPKPLSPLHSLTPYQVPFNAVALRITHSDVAPTHILYAVNASWVGLCKIQDDVRGYTNGPILLAQTPICDCLGFGICRGIDMEKRLYHILTPVPPEELRTVNCLLVGAIAIPHCVLKCQRGIEGTVPYVTTDYNFKLPGASEKIGAREPEEAHKEKPYRRPKFCRKMK

相同性解析による交差性が予測される生物種
Full Name nucleolar protein 9
Calculated molecular weight 702 aa, 79 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC009257
Gene Symbol NOL9
Gene ID (NCBI) 79707
RRIDAB_11124314
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SY16
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NOL9, also named as Polynucleotide 5'-hydroxyl-kinase NOL9, is a 702 amino acid protein, which belongs to the Clp1 family. NOL9 localizes in the nucleus and as a polynucleotide 5'-kinase is involved in rRNA processing. its polynucleotide kinase activity is required for efficient generation of the 5.8S and 28S rRNAs from the 32S precursor.

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