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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NOL7 Polyclonal antibody (18424-1-AP)

    WB analysis of HEK-293 using 18424-1-AP

    WB result of NOL7 antibody (18424-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NOL7 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using NOL7 Polyclonal antibody (18424-1-AP)

    WB analysis using 18424-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18424-1-AP (NOL7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  • KD/KO Validated

NOL7 Polyclonal antibody

NOL7 Polyclonal Antibody for WB, ELISA
Cat No. 18424-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C6orf90, NOP27, Nucleolar protein 7, nucleolar protein 7, 27kDa, Nucleolar protein of 27 kDa

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18424-1-AP targets NOL7 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NOL7 fusion protein Ag13272 相同性解析による交差性が予測される生物種
Full Name nucleolar protein 7, 27kDa
Calculated molecular weight 257 aa, 29 kDa
Observed molecular weight38 kDa
GenBank accession numberBC023517
Gene Symbol NOL7
Gene ID (NCBI) 51406
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UMY1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOL7 (nucleolar protein 7) is localized in both the nucleolus and nucleoplasm, with its nucleolar localization being RNA-dependent. Its function in the nucleolus is likely related to maintaining nucleolar structure and cell growth, while its role in the nucleoplasm may be associated with anti-angiogenic effects. Additionally, NOL7 is involved in the accumulation of early pre-rRNA and the processing of pre-18S rRNA, which are crucial for maintaining nucleolar function and cell growth. The expression of NOL7 is regulated by various transcription factors, including c-myc and RXRα. In cervical cancer, NOL7 transcription is also positively regulated by the RB tumor suppressor gene. The NOL7 gene encodes three distinct splice variants, each with different intracellular localizations that may affect their functions.

Protocols

Product Specific Protocols
WB protocol for NOL7 antibody 18424-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols