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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOL12 Polyclonal antibody (15456-1-AP)

    WB analysis using 15456-1-AP (same clone as 15456-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NOL12 Polyclonal antibody (15456-1-AP)

    WB analysis of HeLa using 15456-1-AP (same clone as 15456-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using NOL12 Polyclonal antibody (15456-1-AP)

    WB analysis of SH-SY5Y using 15456-1-AP (same clone as 15456-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NOL12 Polyclonal antibody (15456-1-AP)

    WB analysis of HepG2 using 15456-1-AP (same clone as 15456-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15456-1-AP (NOL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using NOL12 Polyclonal antibody (15456-1-AP)

    IP experiment of HeLa using 15456-1-AP (same clone as 15456-1-PBS)

    IP result of anti-NOL12 (IP:15456-1-AP, 4ug; Detection:15456-1-AP 1:300) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NOL12 Polyclonal antibody (15456-1-AP)

    IF Staining of HepG2 using 15456-1-AP (same clone as 15456-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NOL12 antibody (15456-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15456-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NOL12 Polyclonal antibody, PBS Only

NOL12 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 15456-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15456-1-PBS targets NOL12 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7723

Product name: Recombinant human NOL12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-213 aa of BC002808

Sequence: MGRNKKKKRDGDDRRPRLVLSFDEEKRREYLTGFHKRKVERKKAAIEEIKQRLKEEQRKLREERHQEYLKMLAEREEALEEADELDRLVTAKTESVQYDHPNHTVTVTTISDLDLSGARLLGLTPPEGGAGDRSEEEASSTEKPTKALPRKSRDPLLSQRISSLTASLHAHSRKKVKRKHPRRAQDSKKPPRAPRTSKAQRRRLTGKARHSGE

相同性解析による交差性が予測される生物種
Full Name nucleolar protein 12
Calculated molecular weight 25 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC002808
Gene Symbol NOL12
Gene ID (NCBI) 79159
RRIDAB_2282718
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGY1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Nucleolar protein 12 (NOL12), a multifunctional RNA-binding protein, has been found to be related to genomic stability, DNA damage repair, and apoptosis. High expression of NOL12 is associated with worse reduced overall survival (OS), high pathological grade, node metastasis, and advanced clinical stage in patients with HCC (PMID: 35693698). Knockdown of NOL12 impairs cellular proliferation by inducing a G1/S cell cycle arrest, but does so outside of a nucleolar stress response and in a p53-independent manner (PMID: 29069457).

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