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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NOL11 Recombinant antibody (83391-6-RR)

    WB analysis using 83391-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83391-6-RR (NOL11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NOL11 Recombinant antibody (83391-6-RR)

    IF Staining of HeLa using 83391-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOL11 antibody (83391-6-RR, Clone: 240371B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NOL11 Recombinant antibody (83391-6-RR)

    IF Staining of HeLa using 83391-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NOL11 antibody (83391-6-RR, Clone: 240371B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using NOL11 Recombinant antibody (83391-6-RR)

    FC experiment of HeLa using 83391-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NOL11 Recombinant antibody (83391-6-RR, Clone:240371B5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83391-6-RR

    Affinity and Kinetic Characterization of 83391-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83391-6-RR against Human NOL11 were performed. The affinity constant is below 1 pM.

NOL11 Recombinant monoclonal antibody

NOL11 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83391-6-RR
Clone No.240371B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Nucleolar protein 11, L14, 240371B5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HT-29 cells, SW480 cells, A431 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83391-6-RR targets NOL11 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag11783

Product name: Recombinant human NOL11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC064404

Sequence: MAALEEEFTLSSVVLSAGPEGLLGVEQSDKTDQFLVTDSGRTVILYKVSDQKPLGSWSVKQGQIITCPAVCNFQTGEYVVVHDNKVLRIWNNEDVNLDKVFKATLSAEVYRILSVQGTEPLVLFKEGAVRGLEALLADPQQKIETVISDEEVIKWTKFFVVFRHPVLIFITEKHGNYFAYVQMFNSRILTKYTLLLGQDENSVIKSFTASVDRKFISLMSLSSDGCIYETLIPIRPADPEKNQSLVKSLLLKAVVSGNARNGVALTALDQDHVAVLGSPLAASKECLSVWNIKFQTLQTSKELPQGTSGQLWYYGEHLFMLHGKSLTVIPYKCEVSSLAGALGKLKHSQDP

相同性解析による交差性が予測される生物種
Full Name nucleolar protein 11
Calculated molecular weight 719 aa, 81 kDa
Observed molecular weight81 kDa
GenBank accession numberBC064404
Gene Symbol NOL11
Gene ID (NCBI) 25926
RRIDAB_3671045
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9H8H0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NOL11, also named as L14, is a ribosome biogenesis factor. It is required for both optimal rDNA transcription and small subunit (SSU) pre-rRNA processing at sites A', A0, 1 and 2b. NOL11 forms a protein complex with two tryptophan-aspartic acid (WD) repeat proteins named WD-repeat protein 43 (WDR43) and Cirhin in mitotic cells. NOL11 plays a role in pathogenesis of NAIC. There're some isoforms with MW 81 kDa and 19 kDa.

Protocols

Product Specific Protocols
WB protocol for NOL11 antibody 83391-6-RRDownload protocol
IF protocol for NOL11 antibody 83391-6-RRDownload protocol
FC protocol for NOL11 antibody 83391-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols