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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NODAL Polyclonal antibody (27430-1-AP)

    WB analysis of HeLa using 27430-1-AP (same clone as 27430-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 27430-1-AP (NODAL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NODAL Polyclonal antibody (27430-1-AP)

    IHC staining of human stomach cancer using 27430-1-AP (same clone as 27430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NODAL Polyclonal antibody (27430-1-AP)

    IHC staining of human stomach cancer using 27430-1-AP (same clone as 27430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using NODAL Polyclonal antibody (27430-1-AP)

    IHC staining of human endometrial cancer using 27430-1-AP (same clone as 27430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using NODAL Polyclonal antibody (27430-1-AP)

    IHC staining of human endometrial cancer using 27430-1-AP (same clone as 27430-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 27430-1-AP (NODAL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NODAL Polyclonal antibody (27430-1-AP)

    IF Staining of HeLa using 27430-1-AP (same clone as 27430-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NODAL antibody (27430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NODAL Polyclonal antibody (27430-1-AP)

    IF Staining of HeLa using 27430-1-AP (same clone as 27430-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NODAL antibody (27430-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293T cells using NODAL Polyclonal antibody (27430-1-AP)

    FC experiment of HEK-293T using 27430-1-AP (same clone as 27430-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human NODAL (27430-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 27430-1-PBS in a different storage buffer formulation.

NODAL Polyclonal antibody, PBS Only

NODAL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 27430-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

nodal homolog (mouse), Nodal homolog

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27430-1-PBS targets NODAL in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26729

Product name: Recombinant human NODAL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 230-347 aa of BC104976

Sequence: EWGKRHRRHHLPDRSQLCRKVKFQVDFNLIGWGSWIIYPKQYNAYRCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLDHHKDMIVEECGCL

相同性解析による交差性が予測される生物種
Full Name nodal homolog (mouse)
Calculated molecular weight 347 aa, 40 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC104976
Gene Symbol NODAL
Gene ID (NCBI) 4838
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96S42
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Nodal is a member of the transforming growth factor-β (TGF-β) superfamily that plays critical roles during embryogenesis. Nodal also regulates cell proliferation, apoptosis, and invasion in cancer cells. However, it appears to exert both tumor-suppressing and tumor-promoting effects, depending on the cell type (PMID: 22645523). Nodal signals through ALK7 receptor to inhibit proliferation and to induce apoptosis in ovarian cancer cell lines and breast cancer cell lines (PMID: 15531507, PMID: 21383881).

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