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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NNMT Recombinant monoclonal antibody (84876-9-RR)

    WB analysis using 84876-9-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84876-9-RR (NNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NNMT Recombinant monoclonal antibody (84876-9-RR)

    IHC staining of human colon cancer using 84876-9-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84876-9-RR (NNMT antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NNMT Recombinant monoclonal antibody (84876-9-RR)

    IHC staining of human colon cancer using 84876-9-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84876-9-RR (NNMT antibody) at dilution of 1:5000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NNMT Recombinant monoclonal antibody (84876-9-RR)

    IF Staining of HepG2 using 84876-9-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NNMT antibody (84876-9-RR, Clone: 242394A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 84876-9-RR

    Affinity of 84876-9-RR

    Biolayer interferometry (BLI) kinetic assays of 84876-9-RR against Human NNMT were performed. The affinity constant is 10.3 nM.

NNMT Recombinant monoclonal antibody

NNMT Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84876-9-RR
Clone No.242394A8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:2.1.1.1, Nicotinamide N-methyltransferase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inL02 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84876-9-RR targets NNMT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7197

Product name: Recombinant human NNMT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-264 aa of BC000234

Sequence: MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL

相同性解析による交差性が予測される生物種
Full Name nicotinamide N-methyltransferase
Calculated molecular weight 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC000234
Gene Symbol NNMT
Gene ID (NCBI) 4837
RRIDAB_3743801
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP40261
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NNMT can catalyze the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway (PMID: 21823666; 23455543; 8182091). It plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3 (PMID: 23455543; PMID: 26571212; PMID: 31043742).

Protocols

Product Specific Protocols
IF protocol for NNMT antibody 84876-9-RRDownload protocol
IHC protocol for NNMT antibody 84876-9-RRDownload protocol
WB protocol for NNMT antibody 84876-9-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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