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Validation Data Gallery

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  • Western Blot (WB) analysis of human colon tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    WB analysis of human colon using 14619-1-AP (same clone as 14619-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 14619-1-AP (NMUR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14619-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    WB analysis of mouse pancreas using 14619-1-AP (same clone as 14619-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14619-1-AP (NMUR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14619-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human stomach tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    WB analysis of human stomach using 14619-1-AP (same clone as 14619-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 14619-1-AP (NMUR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14619-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    IHC staining of mouse brain using 14619-1-AP (same clone as 14619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14619-1-AP (NMUR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14619-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    IHC staining of mouse brain using 14619-1-AP (same clone as 14619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14619-1-AP (NMUR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14619-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    IHC staining of human testis using 14619-1-AP (same clone as 14619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14619-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    IHC staining of human testis using 14619-1-AP (same clone as 14619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14619-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    IHC staining of human colon using 14619-1-AP (same clone as 14619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14619-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using NMUR1 Polyclonal antibody (14619-1-AP)

    IHC staining of human colon using 14619-1-AP (same clone as 14619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14619-1-AP (NMUR1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14619-1-PBS in a different storage buffer formulation.

NMUR1 Polyclonal antibody, PBS Only

NMUR1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14619-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14619-1-PBS targets NMUR1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6235

Product name: Recombinant human NMUR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 119-426 aa of BC036543

Sequence: YEMWHNYPFLLGVGGCYFRTLLFEMVCLASVLNVTALSVERYVAVVHPLQARSMVTRAHVRRVLGAVWGLAMLCSLPNTSLHGIQQLHVPCRGPVPDSAVCMLVRPRALYNMVVQTTALLFFCLPMAIMSVLYLLIGLRLRRERLLLMQEAKGRGSAAARSRYTCRLQQHDRGRRQVTKMLFVLVVVFGICWAPFHADRVMWSVVSQWTDGLHLAFQHVHVISGIFFYLGSAANPVLYSLMSSRFRETFQEALCLGACCHRLRPRHSSHSLSRMTTGSTLCDVGSLGSWVHPLAGNDGPEAQQETDPS

相同性解析による交差性が予測される生物種
Full Name neuromedin U receptor 1
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC036543
Gene Symbol NMUR1
Gene ID (NCBI) 10316
RRIDAB_2267310
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HB89
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Neuromedin-U (NMU) is a widely expressed peptide with the highest abundance in the central nervous system (CNS) and intestinal tract of nearly all vertebrate species. NMU mediates several physiological functions via its two cognate receptors, NMUR1 and NMUR2 (PMID:37102164). NMUR1(NmU receptor 1) has the highest expression in peripheral tissues, particularly the GI tract, pancreas, uterus, and testes but has also been reported in peripheral nerve terminals (PMID:10783389).

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