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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NMI Recombinant antibody (83986-2-RR)

    WB analysis of HeLa using 83986-2-RR

    WB result of NMI antibody (83986-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NMI transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using NMI Recombinant antibody (83986-2-RR)

    WB analysis using 83986-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83986-2-RR (NMI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NMI Recombinant antibody (83986-2-RR)

    IF Staining of HepG2 using 83986-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NMI antibody (83986-2-RR, Clone: 241135A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using NMI Recombinant antibody (83986-2-RR)

    FC experiment of HepG2 using 83986-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NMI Recombinant antibody (83986-2-RR, Clone:241135A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 83986-2-RR

    Affinity and Kinetic Characterization of 83986-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83986-2-RR against Human NMI were performed. The affinity constant is 1.98 nM.

  • Featured Product
  • KD/KO Validated

NMI Recombinant monoclonal antibody

NMI Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83986-2-RR
Clone No.241135A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

241135A9, N myc (and STAT) interactor, N myc and STAT interactor, N myc interactor, N-myc and STAT interactor

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, PC-3 cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83986-2-RR targets NMI in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34759

Product name: Recombinant human NMI protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of BC001268

Sequence: MEADKDDTQQILKEHSPDEFIKDEQNKGLIDEITKKNIQLKKEIQKLETELQEATKEFQIKEDIPETKMKFLSVETPENDSQLSNISCSFQVSSKVPYEI

相同性解析による交差性が予測される生物種
Full Name N-myc (and STAT) interactor
Calculated molecular weight 35 kDa
Observed molecular weight35 kDa
GenBank accession numberBC001268
Gene Symbol NMI
Gene ID (NCBI) 9111
RRIDAB_3671560
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13287
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

N-Myc and STAT Interactor protein (NMI) is an interferon inducible protein that interacts with NMYC and CMYC (members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. It is widely involved in the process of tumor growth, progression, and metastasis.

Protocols

Product Specific Protocols
IF protocol for NMI antibody 83986-2-RRDownload protocol
WB protocol for NMI antibody 83986-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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