Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32694-1-AP targets NLRP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38545 Product name: Recombinant human NLRP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 11-200 aa of NM_134444.4 Sequence: LMWYLEELKKEEFRKFKEHLKQMTLQLELKQIPWTEVKKASREELANLLIKHYEEQQAWNITLRIFQKMDRKDLCMKVMRERTGYTKTYQAHAKQKFSRLWSSKSVTEIHLYFEEEVKQEECDHLDRLFAPKEAGKQPRTVIIQGPQGIGKTTLLMKLMMAWSDNKIFRDRFLYTFYFCCRELRELPPTS 相同性解析による交差性が予測される生物種 |
| Full Name | NLR family, pyrin domain containing 4 |
| Calculated molecular weight | 113kDa，994aa |
| Observed molecular weight | 98-105 kDa |
| GenBank accession number | NM_134444.4 |
| Gene Symbol | NLRP4 |
| Gene ID (NCBI) | 147945 |
| RRID | AB_3742711 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96MN2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NLRP4 is a member of the NOD-like receptor (NLR) family, a group of intracellular pattern recognition receptors (PRRs) that play crucial roles in innate immunity, inflammation, and cell death regulation.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NLRP4 antibody 32694-1-AP | Download protocol |
| WB protocol for NLRP4 antibody 32694-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |