Validation Data Gallery
Tested Applications
| Positive WB detected in | Daudi cells, Jurkat cells, Raji cells, Ramos cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30455-1-AP targets NLRC3 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32323 Product name: Recombinant human NLRC3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-130 aa of NM_178844 Sequence: MRKQEVRTGREAGQGHGTGSPAEQVKALMDLLAGKGSQGSQAPQALDRTPDAPLGPCSNDSRIQRHRKALLSKVGGGPELGGPWHRLASLLLVEGLTDLQLREHDFTQVEATRGGGHPARTVALDRLFLP 相同性解析による交差性が予測される生物種 |
| Full Name | NLR family, CARD domain containing 3 |
| Calculated molecular weight | 115 kDa |
| Observed molecular weight | 100-110 kDa |
| GenBank accession number | NM_178844 |
| Gene Symbol | NLRC3 |
| Gene ID (NCBI) | 197358 |
| RRID | AB_3086322 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7RTR2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NLRC3 is a member of the pattern recognition receptors nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) family, and plays a pivotal regulatory role in modulating the activation of immune cells. In macrophages, NLRC3 inhibits the activation of the NF-κB signaling pathway, the STING/TBK1 signaling pathway, and the formation of the inflammasome. In the context of T cells immune response, NLRC3 prevents the activation of T cells by regulating the function of dendritic cells and directly influencing the function of T cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NLRC3 antibody 30455-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |