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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse ovary tissue using NLN Polyclonal antibody (14763-1-AP)

    WB analysis of mouse ovary using 14763-1-AP (same clone as 14763-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14763-1-AP (NLN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14763-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NLN Polyclonal antibody (14763-1-AP)

    WB analysis of HepG2 using 14763-1-AP (same clone as 14763-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14763-1-AP (NLN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NLN Polyclonal antibody (14763-1-AP)

    IHC staining of mouse testis using 14763-1-AP (same clone as 14763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14763-1-AP (NLN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14763-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NLN Polyclonal antibody (14763-1-AP)

    IHC staining of mouse testis using 14763-1-AP (same clone as 14763-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14763-1-AP (NLN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14763-1-PBS in a different storage buffer formulation.

NLN Polyclonal antibody, PBS Only

NLN Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14763-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

AGTBP, Angiotensin binding protein, Angiotensin-binding protein, EC:3.4.24.16, KIAA1226

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14763-1-PBS targets NLN in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6524

Product name: Recombinant human NLN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 289-607 aa of BC001644

Sequence: KVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAEREFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEGLLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICAQVSFFFPPVNLPISFFRKLLDCCQVSAPNRFFQKLNVFKNFIGLLQNQNFPYPVLFHNIFLLGGSSTSERCLLNNSLWKKNKTICKERLQILCFFLLIL

相同性解析による交差性が予測される生物種
Full Name neurolysin (metallopeptidase M3 family)
Calculated molecular weight 81 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC001644
Gene Symbol NLN
Gene ID (NCBI) 57486
RRIDAB_2151973
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYT8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NLN(Neurolysin, mitochondrial) is also named as AGTBP, KIAA1226 and belongs to the peptidase M3 family. It is an enzyme involved in the metabolic inactivation of bioactive peptides and functions as a monomer of molecular mass 78 kDa. It also can be dected the molecular weight between 70 kDa and 85 kDa according to the reports (PMID:10095765, 11092934). The full length protein has a transit peptide with 37 amino acids.

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