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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse ovary tissue using NLN Polyclonal antibody (14763-1-AP)

    WB analysis of mouse ovary using 14763-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14763-1-AP (NLN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NLN Polyclonal antibody (14763-1-AP)

    WB analysis of HepG2 using 14763-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14763-1-AP (NLN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NLN Polyclonal antibody (14763-1-AP)

    IHC staining of mouse testis using 14763-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14763-1-AP (NLN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NLN Polyclonal antibody (14763-1-AP)

    IHC staining of mouse testis using 14763-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14763-1-AP (NLN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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NLN Polyclonal antibody

NLN Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14763-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

AGTBP, Angiotensin binding protein, KIAA1226, MEP, Microsomal endopeptidase, Mitochondrial oligopeptidase M, Neurolysin, mitochondrial, Neurotensin endopeptidase, NLN

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse ovary tissue, HepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14763-1-AP targets NLN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NLN fusion protein Ag6524 相同性解析による交差性が予測される生物種
Full Name neurolysin (metallopeptidase M3 family)
Calculated molecular weight 81 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC001644
Gene Symbol NLN
Gene ID (NCBI) 57486
RRIDAB_2151973
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYT8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NLN(Neurolysin, mitochondrial) is also named as AGTBP, KIAA1226 and belongs to the peptidase M3 family. It is an enzyme involved in the metabolic inactivation of bioactive peptides and functions as a monomer of molecular mass 78 kDa. It also can be dected the molecular weight between 70 kDa and 85 kDa according to the reports (PMID:10095765, 11092934). The full length protein has a transit peptide with 37 amino acids.

Protocols

Product Specific Protocols
WB protocol for NLN antibody 14763-1-APDownload protocol
IHC protocol for NLN antibody 14763-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols