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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NLGN4X Polyclonal antibody (27278-1-AP)

    WB analysis using 27278-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27278-1-AP (NLGN4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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NLGN4X Polyclonal antibody

NLGN4X Polyclonal Antibody for WB, ELISA
Cat No. 27278-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

ASPGX2, AUTSX2, HLNX, HNLX, KIAA1260, neuroligin 4, X linked, Neuroligin X, NLGN, NLGN4, NLGN4X

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27278-1-AP targets NLGN4X in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NLGN4X fusion protein Ag2426 相同性解析による交差性が予測される生物種
Full Name neuroligin 4, X-linked
Calculated molecular weight 92 kDa
Observed molecular weight90-100 kDa
GenBank accession numberBC034018
Gene Symbol NLGN4X
Gene ID (NCBI) 57502
RRIDAB_3085943
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N0W4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NLGN4X is a member of the type-B carboxylesterase/lipase protein family. The encoded protein belongs to a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. The encoded protein interacts with discs large homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. NLGN4X has two isoforms with predicted MW's of 92 kDa and 94 kDa.

Protocols

Product Specific Protocols
WB protocol for NLGN4X antibody 27278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols