Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, U-87 MG cells, rat brain tissue, U-251 cells, SH-SY5Y cells, fetal human brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87774-2-RR targets NLGN3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg5477 Product name: Recombinant human NLGN3 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 38-709 aa of NM_181303 Sequence: QAPAPTVNTHFGKLRGARVPLPSEILGPVDQYLGVPYAAPPIGEKRFLPPEPPPSWSGIRNATHFPPVCPQNIHTAVPEVMLPVWFTANLDIVATYIQEPNEDCLYLNVYVPTEDVKRISKECARKPNKKICRKGGSGAKKQGEDLADNDGDEDEDIRDSGAKPVMVYIHGGSYMEGTGNMIDGSILASYGNVIVITLNYRVGVLGFLSTGDQAAKGNYGLLDQIQALRWVSENIAFFGGDPRRITVFGSGIGASCVSLLTLSHHSEGLFQRAIIQSGSALSSWAVNYQPVKYTSLLADKVGCNVLDTVDMVDCLRQKSAKELVEQDIQPARYHVAFGPVIDGDVIPDDPEILMEQGEFLNYDIMLGVNQGEGLKFVEGVVDPEDGVSGTDFDYSVSNFVDNLYGYPEGKDTLRETIKFMYTDWADRDNPETRRKTLVALFTDHQWVEPSVVTADLHARYGSPTYFYAFYHHCQSLMKPAWSDAAHGDEVPYVFGVPMVGPTDLFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNKPVPQDTKFIHTKANRFEEVAWSKYNPRDQLYLHIGLKPRVRDHYRATKVAFWKHLVPHLYNLHDMFHYTSTTTKVPPPDTTHSSHITRRPNGKTWSTKRPAISPAYSNENAQGSWNGDQDAGPLLVENPRDYSTELS 相同性解析による交差性が予測される生物種 |
| Full Name | neuroligin 3 |
| Calculated molecular weight | 94 kDa |
| Observed molecular weight | 90 kDa, 110 kDa |
| GenBank accession number | NM_181303 |
| Gene Symbol | NLGN3 |
| Gene ID (NCBI) | 54413 |
| RRID | AB_3745747 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NZ94-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Neuroligins (NLGNs) are cell adhesion molecules located on the postsynaptic side of the synapse that interact with their presynaptic partners neurexins to maintain trans-synaptic connection. Three highly homologous neuroligins are abundantly expressed in rodent brain: NLGN1, NLGN2, NLGN3, while NLGN4 is less abundant in rodent brain and less evolutionarily conserved among all NLGNs isoforms. The literature indicates that significantly elevated basal levels of cross-linked NLGN3-positive dimers (sNLGN3, ~300 kDa) can be detected on the synaptic surface of *Fmr1* KO mice. And also Intracellular NLGN3 (iNGLN3 at about 120 kDa) level was increased in basal state Fmr1 KO synaptoneurosomes (PMID: 30056576).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NLGN3 antibody 87774-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |