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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NLGN3 Polyclonal antibody (29319-1-AP)

    WB analysis using 29319-1-AP (same clone as 29319-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29319-1-AP (NLGN3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29319-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NLGN3 Polyclonal antibody (29319-1-AP)

    IHC staining of mouse brain using 29319-1-AP (same clone as 29319-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29319-1-AP (NLGN3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29319-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NLGN3 Polyclonal antibody (29319-1-AP)

    IHC staining of mouse brain using 29319-1-AP (same clone as 29319-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29319-1-AP (NLGN3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29319-1-PBS in a different storage buffer formulation.

NLGN3 Polyclonal antibody, PBS Only

NLGN3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29319-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

NL3, Neuroligin-3, neuroligin 3, HNL3, AUTSX1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29319-1-PBS targets NLGN3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30536

Product name: Recombinant human NLGN3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 38-142 aa of BC051715

Sequence: QAPAPTVNTHFGKLRGARVPLPSEILGPVDQYLGVPYAAPPIGEKRFLPPEPPPYWSGIRNATHFPPVCPQNIHTAVPEVMLPVWFTANLDIVATYIQEPNEDCL

相同性解析による交差性が予測される生物種
Full Name neuroligin 3
Calculated molecular weight 94 kDa
Observed molecular weight90-110 kDa
GenBank accession numberBC051715
Gene Symbol NLGN3
Gene ID (NCBI) 54413
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZ94
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Neuroligin3 (NLGN3) belongs to the neuroligin family, a class of postsynaptic cell adhesion molecules that regulate synapse organization and dendritic outgrowth, and NLGN3 missense variants have previously been associated with autistic spectrum disorder (ASD).

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