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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human NKp46/NCR1 Rabbit Recombinant Antibody (98340-1-RR)

    FC experiment of human PBMCs using 98340-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human NKp46/NCR1 Rabbit RecAb (98340-1-RR, Clone: 242549D12) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human NCAM1/CD56. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human NKp46/NCR1 Rabbit Recombinant Antibody (98340-1-RR)

    FC experiment of human PBMCs using 98340-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human NKp46/NCR1 Rabbit RecAb (98340-1-RR, Clone: 242549D12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Anti-Human NKp46/NCR1 Rabbit Recombinant Antibody

NKp46/NCR1 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98340-1-RR
Clone No.242549D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

NCR 1, Natural killer cell p46-related protein, LY94, hNKp46, CD335

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98340-1-RR targets NKp46/NCR1 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name natural cytotoxicity triggering receptor 1
Calculated molecular weight34kDa
Gene Symbol NCR1
Gene ID (NCBI) 9437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO76036-1
Storage Buffer PBS with 0.09% sodium azide , pH 7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals (PMID: 22021440). NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail (PMID: 9730896). It is expressed on NK cells, rare T-cell subsets and a mucosal population of NKp46+ innate lymphoid cells (PMID: 9730896; 22021440). NKp46 is the major triggering receptor involved in the natural cytotoxicity (PMID: 10359120; 10092106; 15356098).

Protocols

Product Specific Protocols
FC protocol for NKp46/NCR1 antibody 98340-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols