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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using NKX2-5 Polyclonal antibody (13921-1-AP)

    WB analysis of human heart using 13921-1-AP (same clone as 13921-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 13921-1-AP (NKX2-5 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using NKX2-5 Polyclonal antibody (13921-1-AP)

    WB analysis of human heart using 13921-1-AP (same clone as 13921-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 13921-1-AP (NKX2-5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using NKX2-5 Polyclonal antibody (13921-1-AP)

    IHC staining of human brain using 13921-1-AP (same clone as 13921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 13921-1-AP (NKX2-5 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NKX2-5 Polyclonal antibody (13921-1-AP)

    IHC staining of mouse heart using 13921-1-AP (same clone as 13921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13921-1-AP (NKX2-5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using NKX2-5 Polyclonal antibody (13921-1-AP)

    IHC staining of mouse heart using 13921-1-AP (same clone as 13921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13921-1-AP (NKX2-5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using NKX2-5 Polyclonal antibody (13921-1-AP)

    IHC staining of human brain using 13921-1-AP (same clone as 13921-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 13921-1-AP (NKX2-5 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse embryo tissue using NKX2-5 Polyclonal antibody (13921-1-AP)

    IF Staining of mouse embryo using 13921-1-AP (same clone as 13921-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using NKX2-5 antibody (13921-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13921-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

NKX2-5 Polyclonal antibody, PBS Only

NKX2-5 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 13921-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13921-1-PBS targets NKX2-5 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4930

Product name: Recombinant human NKX2-5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-324 aa of BC025711

Sequence: MFPSPALTPTPFSVKDILNLEQQQRSLAAAGELSARLEATLAPSSCMLAAFKPEAYAGPEAAAPGLPELRAELGRAPSPAKCASAFPAAPAFYPRAYSDPDPAKDPRAEKKELCALQKAVELEKTEADNAERPRARRRRKPRVLFSQAQVYELERRFKQQRYLSAPERDQLASVLKLTSTQVKIWFQNRRYKCKRQRQDQTLELVGLPPPPPPPARRIAVPVLVRDGKPCLGDSAPYAPAYGVGLNPYGYNAYPAYPGYGGAACSPGYSCTAAYPAGPSPAQPATAAANNNFVNFGVGDLNAVQSPGIPQSNSGVSTLHGIRAW

相同性解析による交差性が予測される生物種
Full Name NK2 transcription factor related, locus 5 (Drosophila)
Calculated molecular weight 324 aa, 35 kDa
Observed molecular weight30-42 kda
GenBank accession numberBC025711
Gene Symbol NKX2-5
Gene ID (NCBI) 1482
RRIDAB_1851479
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP52952
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development [PMID: 7665173]. NKX2-5 encoded by homeobox-containing genes that implicated in commitment to and/or differentiation of the myocardial lineage, and acts as a transcriptional activator of ANF in cooperation with GATA4. During embryonic development, NKX2-5 plays pivotal roles in specifying cardiac progenitors, cardiac morphogenesis, cardiomyocyte differentiation and conduction system development [PMID: 22824467].

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