Validation Data Gallery
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Tested Applications
| Positive WB detected in | Min6 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IF | See 3 publications below |
| ChIP | See 1 publications below |
Product Information
13013-1-AP targets NKX2-2 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4069 Product name: Recombinant human NKX2-2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-129 aa of BC075092 Sequence: MSLTNTKTGFSVKDILDLPDTNDEEGSVAEGPEEENEGPEPAKRAGPLGQGALDAVQSLPLKNPFYDSSDNPYTRWLASTEGLQYSLHGLAAGAPPQDSSSKSPEPSADESPDNDKETPGGGGDAGKKR 相同性解析による交差性が予測される生物種 |
| Full Name | NK2 homeobox 2 |
| Calculated molecular weight | 273 aa, 30 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC075092 |
| Gene Symbol | NKX2-2 |
| Gene ID (NCBI) | 4821 |
| RRID | AB_2877903 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95096 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NKX2-2 antibody 13013-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Loss of the USP22 deubiquitylase confers resistance to chemotherapy in small cell lung cancer. | ||
Nucleic Acids Res EWS-FLI1 regulates and cooperates with core regulatory circuitry in Ewing sarcoma. | ||
Dev Cell Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination | ||
Dev Cell Identification of signaling pathways that specify a subset of migrating enteric neural crest cells at the wavefront in mouse embryos | ||
Cell Rep A neuronal Slit1-dependent program rescues oligodendrocyte differentiation and myelination under chronic hypoxic conditions |