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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NKIAMRE Polyclonal antibody (13888-1-AP)

    WB analysis of HeLa using 13888-1-AP (same clone as 13888-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13888-1-AP (NKIAMRE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13888-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NKIAMRE Polyclonal antibody (13888-1-AP)

    WB analysis of HeLa using 13888-1-AP (same clone as 13888-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13888-1-AP (NKIAMRE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13888-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using NKIAMRE Polyclonal antibody (13888-1-AP)

    IHC staining of human gliomas using 13888-1-AP (same clone as 13888-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13888-1-AP (NKIAMRE antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13888-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using NKIAMRE Polyclonal antibody (13888-1-AP)

    IHC staining of human gliomas using 13888-1-AP (same clone as 13888-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13888-1-AP (NKIAMRE antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13888-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13888-1-PBS targets NKIAMRE in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NKIAMRE fusion protein Ag4812 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase-like 3
Calculated molecular weight 592 aa, 68 kDa
Observed molecular weight 52-60 kDa
GenBank accession numberBC041799
Gene Symbol NKIAMRE
Gene ID (NCBI) 51265
RRIDAB_2077463
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IVW4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NKIAMRE is also named as CDKL3(Cyclin-dependent kinase-like 3) and belongs to the protein kinase superfamily.This protein contains the MAPK-like TDY motif for threonine and tyrosine phosphorylation, as well as the putative cyclin-binding motif(PMID:10463609). It has 2 isoforms produced by alternative splicing with the molecular mass of 52 kDa and 68 kDa.