Validation Data Gallery
Tested Applications
| Positive WB detected in | NK-92 cells |
| Positive IP detected in | NK-92 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11532-1-AP targets NKG7 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2117 Product name: Recombinant human NKG7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-165 aa of BC015759 Sequence: MELCRSLALLGGSLGLMFCLIALSTDFWFEAVGPTHSAHSGLWPTGHGDIISGYIHVTQTFSIMAVLWALVSVSFLVLSCFPSLFPPGHGPLVSTTAAFAAAISMVVAMAVYTSERWDQPPHPQIQTFFSWSFYLGWVSAILLLCTGALSLGAHCGGPRPGYETL 相同性解析による交差性が予測される生物種 |
| Full Name | natural killer cell group 7 sequence |
| Calculated molecular weight | 165 aa, 18 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | BC015759 |
| Gene Symbol | NKG7 |
| Gene ID (NCBI) | 4818 |
| RRID | AB_3741818 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16617 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Natural killer cell granule protein 7 (NKG7) regulates lymphocyte granule exocytosis and downstream inflammation in a broad range of diseases. NKG7 expressed by CD4+ and CD8+ T cells were key in promoting inflammation during visceral leishmaniasis and malaria-two important parasitic diseases. It is expressed by natural killer cells and is critical for controlling cancer initiation, growth, and metastasis (PMID: 32839608; 8458737). In brief, NKG7 is a necessary T-cell intrinsic factor for the cytotoxic function of CD8+ T cells and established NKG7 as a new therapeutic target for cancer immunotherapy (PMID: 34911739).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NKG7 antibody 11532-1-AP | Download protocol |
| IP protocol for NKG7 antibody 11532-1-AP | Download protocol |
| WB protocol for NKG7 antibody 11532-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |